现代畜牧科技Issue(9)：29-32,4.DOI:10.19369/j.cnki.2095-9737.2025.09.008
猪流行性腹泻病毒阻断ELISA抗体检测试剂盒的制备
Preparation of Porcine Epidemic Diarrhea Virus Blocking ELISA Antibody Detection Kit
摘要
Abstract
In order to develop a highly effective diagnostic reagent for porcine epidemic diarrhoea virus(PEDV),a porcine epidemic diarrhoea virus blocking ELISA antibody detection kit was developed by preparing pre-coated microplates and enzyme labelled antibodies,and specific tests,neutralising activity tests and clinical diagnostic tests were carried out to verify the performance of the kits.The results showed that the monoclonal antibody prepared by the kit had good specificity and only reacted positively with PEDV-related strains,and had no cross-reactivity with other strains and cells.Each monoclonal antibody had high neutralising activity against different PEDV strains,such as PEDV-Mabs-2D5 against PEDV-KB2013-4 strains with a neutralising titre of 1∶2 048,the minimum detection amount of the kit was 1∶256,and the specificity coincidence rate was 100%,and the clinical test results were consistent with expectations.In summary,the ELISA antibody detection kit for swine epidemic diarrhoea virus blockade prepared in this experiment has strong specificity and high sensitivity,which is of great significance for judging the PEDV infection situation in pig farms and evaluating the immunisation status of swine fever vaccine,and provides an effective detection method for PEDV prevention and control.关键词
猪流行性腹泻/特异性试验/ELISA抗体检测Key words
PED/specificity test/ELISA antibody detection分类
农业科技引用本文复制引用
冯平,符美静,张磊,瞿军军,张艳,陈瑞..猪流行性腹泻病毒阻断ELISA抗体检测试剂盒的制备[J].现代畜牧科技,2025,(9):29-32,4.基金项目
陕西省教育厅服务地方专项(19JC043) （19JC043）
榆林学院高层次人才科研启动基金项目(20GK12,20GK13) （20GK12,20GK13）
榆林市科技局产学研合作项目(2019-103-5) （2019-103-5）
