中国免疫学杂志2025，Vol.41Issue(9)：2087-2091,5.DOI:10.3969/j.issn.1000-484X.2025.09.007
类风湿关节炎患者外周血CD4+CD28-T细胞亚群免疫功能相关分子的表达分析
Expression of immune function-related molecules in CD4+CD28-T cell subset in peripheral blood of rheumatoid arthritis patients
摘要
Abstract
Objective:To explore the activation pathway of CD4+CD28-T cells and their specific role in rheumatoid arthritis(RA).Methods:Peripheral blood mononuclear cells(PBMCs)were isolated from RA patients and healthy individuals.Flow cytome-try was used to detect the proportion and activation level of CD4+CD28-T cells,as well as the expressions of perforin and TNF-α,and to analyze the correlation between the proportion of CD4+CD28-T cells and age,C-reactive protein,anti-cyclic citrullinated peptide(CCP)antibodies,rheumatoid factors(RF)and erythrocyte sedimentation rate(ESR).The expression of Toll-like receptor(TLR)2 on CD4+CD28-T cells was detected by flow cytometry.Purified RA CD4+T cells were stimulated with anti-CD3 antibody and TLR2 agonist alone or in combination,and the activation level and cytokine secretion of CD4+CD28-T cells were detected by flow cytometry.Results:The proportion of CD4+CD28-T cells in RA patients was higher than that in healthy individuals(P<0.05),and was positively correlated with rheumatoid factors and erythrocyte sedimentation rate(r=0.554 1,P<0.01;r=0.363 4,P<0.01).Compared with CD4+CD28+T cells,CD25 expression was downregulate(P<0.01),but CD40L and TLR2 expressions were upregulated(P<0.01;P<0.000 1)in CD4+CD28-T cells from RA patients,and they secreted more perforin and TNF-α(P<0.001;P<0.001).After TLR2 ago-nist stimulation,there was no significant change in CD40L and CD25 expression,as well as perforin and TNF-α secretion in CD4+CD28-T cells from RA patients.Conclusion:The proportion of CD4+CD28-T cells is increased in RA patients,and they secrete a large amount of perforin and TNF-α,which may be involved in the inflammatory state and joint damage of RA,but TLR2 cannot re-place CD28 in activating CD4+CD28-T cells.关键词
类风湿关节炎/CD4+CD28-T细胞/Toll样受体2/TNF-α/穿孔素Key words
Rheumatoid arthritis/CD4+CD28-T cells/Toll-like receptor 2/TNF-α/Perforin分类
医药卫生引用本文复制引用
林倩,张诚,张杰,黄会娜,李霞,王冠..类风湿关节炎患者外周血CD4+CD28-T细胞亚群免疫功能相关分子的表达分析[J].中国免疫学杂志,2025,41(9):2087-2091,5.基金项目
国家自然科学基金青年科学基金项目(82101896) （82101896）
国家自然科学基金面上项目(82271839) （82271839）
辽宁省教育厅基本科研青年项目(LJ212410161057) （LJ212410161057）
辽宁省教育厅基本科研重点项目(LJ212410161034). （LJ212410161034）
