中国免疫学杂志2025，Vol.41Issue(9)：2161-2165,2171,6.DOI:10.3969/j.issn.1000-484X.2025.09.018
麻黄碱调节RIP1/RIP3/MLKL信号通路对OGD/R诱导的神经元损伤的影响
Effect of Ephedrine on OGD/R induced neuronal injury by regulating RIP1/RIP3/MLKL signaling pathway
摘要
Abstract
Objective:To explore the effect of Ephedrine on oxygen glucose deprivation/reoxygenation(OGD/R)induced neu-ronal injury by regulating receptor interacting protein-1/receptor interacting protein-3/mixed lineage kinase domain-like protein(RIP1/RIP3/MLKL)signaling pathway.Methods:OGD/R model was constructed by hypoxia/reoxygenation;human cortical neuron HCN cells were separated into control group,model group(OGD/R group),Ephedrine group(800 μg/ml),Compound 6i group(RIP1 acti-vator Compound 6i,0.94 μmol/L),Ephedrine+Compound 6i group(800 μg/ml Ephedrine+0.94 μmol/L Compound 6i),Nec-1 group(RIP1 specific inhibitor Nec-1,5 μmol/L)and Ephedrine+Nec-1 group(800 μg/ml Ephedrine+5 μmol/L Nec-1);CCK-8 method was applied to detect HCN cell proliferation;DCFH-DA method was applied to detect ROS level in HCN cells;flow cytometry was ap-plied to detect HCN cell apoptosis;ELISA was applied to detect levels of TNF-α and IL-6;Western blot was applied to detect RIP1/RIP3/MLKL signaling pathway and apoptotic proteins expressions in HCN cells.Results:Compared with control group,ROS level,apop-tosis rate,p-MLKL,RIP3,Bax,IL-6,TNF-α and RIP1 expressions of HCN cells in OGD/R group were significantly increased,A450 value and Bcl-2 protein were significantly decreased(P<0.05);compared with OGD/R group,ROS level,apoptosis rate,p-MLKL,RIP3,Bax,IL-6,TNF-α and RIP1 expressions of HCN cells in Ephedrine group and Nec-1 group were significantly reduced,A450 val-ue and Bcl-2 protein were significantly increased,the trend of Compound 6i group was opposite(P<0.05);Compound 6i was able to weaken the effect of Ephedrine in improving OGD/R induced neuronal injury,Nec-1 was able to enhance the effect of Ephedrine in im-proving OGD/R induced neuronal injury.Conclusion:Ephedrine attenuates OGD/R induced neuronal injury by inhibiting RIP1/RIP3/MLKL signaling pathway.关键词
麻黄碱/RIP1/RIP3/MLKL/OGD/R/增殖/凋亡/神经元Key words
Ephedrine/RIP1/RIP3/MLKL/OGD/R/Proliferation/Apoptosis/Neuron分类
医药卫生引用本文复制引用
李鹏,李爱歆,郭思佳..麻黄碱调节RIP1/RIP3/MLKL信号通路对OGD/R诱导的神经元损伤的影响[J].中国免疫学杂志,2025,41(9):2161-2165,2171,6.基金项目
黑龙江省教育厅基本科研业务费基础研究项目(2021-KYYWF-0608). （2021-KYYWF-0608）
