中国免疫学杂志2025，Vol.41Issue(9)：2166-2171,6.DOI:10.3969/j.issn.1000-484X.2025.09.019
仙茅苷调节cGAS-STING信号通路对急性脑梗死大鼠神经元焦亡的影响
Impact of curculigoside on neuronal pyroptosis in rats with acute cerebral infarction by regulating cGAS-STING signaling pathway
摘要
Abstract
Objective:To explore effect of curculigoside on neuronal pyroptosis in rats with acute cerebral infarction(CI)by regulating cyclic guanosine-adenosine synthase(cGAS)-interferon gene stimulating factor(STING)signaling pathway.Methods:Fifteen rats were randomly selected as sham operation group,and remaining rats were constructed CI models by modified Longa suture method,CI rats successfully modeled were randomly divided into CI group,curculigoside group(5 mg/kg),SR-717 group(3 mg/kg cGAS-STING signaling pathway activator SR-717)and curculigoside+SR-717 group(5 mg/kg curculigoside+3 mg/kg SR-717),with 15 rats in each group,injected once a day for 7 consecutive days,sham operation group and CI group were given equal amounts of nor-mal saline.Neural function was evaluated by Zea-Longa score;inflammatory factors levels were detected by ELISA;TTC staining was used to evaluate volume of CI;TUNEL staining was used to detect neuronal apoptosis;immunofluorescence staining was used to detect GSDMD-N expression;Western blot was used to detect expression of pyroptosis and cGAS-STING pathway proteins.Results:Com-pared with sham operation group,Zea-Longa score,CI volume,IL-1β and IL-18 levels,apoptosis rate,number of GSDMD-N positive cells,NLRP3,cleaved-Caspase-1/Caspase-1,GSDMD-N,cGAS and STING protein expressions in CI group were significantly increased(P<0.05);compared with CI group,Zea-Longa score,CI volume,IL-1β and IL-18 levels,apoptosis rate,number of GSDMD-N positive cells,NLRP3,cleaved-Caspase-1/Caspase-1,GSDMD-N,cGAS and STING protein expressions in curculigoside group were significantly decreased(P<0.05),while the above indexes in SR-717 group had opposite trend(P<0.05);SR-717 reversed improvement effect of curculigoside on neuronal pyroptosis in CI rats.Conclusion:Curculigoside may improve neuronal pyroptosis in CI rats by down-regulating cGAS-STING signaling pathway.关键词
仙茅苷/cGAS-STING信号通路/急性脑梗死/神经元/焦亡Key words
Curculigoside/cGAS-STING signaling pathway/Acute cerebral infarction/Neuron/Pyroptosis分类
医药卫生引用本文复制引用
段姝洁,龚清源,王彦丽,戴启荷..仙茅苷调节cGAS-STING信号通路对急性脑梗死大鼠神经元焦亡的影响[J].中国免疫学杂志,2025,41(9):2166-2171,6.基金项目
2019年荆州市科教计划项目(2019HC03). （2019HC03）
