中国水稻科学2025，Vol.39Issue(5)：615-623,9.DOI:10.16819/j.1001-7216.2025.240510
水稻OsAlR1基因耐铝性功能研究
Functional Study on Aluminum Tolerance of OsAlR1 Gene in Rice
摘要
Abstract
[Objective]With the aggravation of soil acidification,aluminum(Al)toxicity in acidic soil seriously limits the growth and development of rice.Mining Al-tolerant gene resources,analyzing their molecular functions,and breeding Al-tolerant varieties can ensure rice yield in acidic soil.[Method]CRISPR/Cas9 technology was used to construct knockout vectors.The overexpression vector was constructed by homologous recombination and Golden Gate seamless cloning.Transgenic materials were obtained by genetic transformation.Homozygous overexpression lines and knockout lines were screened for Al tolerance phenotype analysis and determination of reactive oxygen species(ROS)content and antioxidant enzyme activities.[Result]qRT-PCR results showed that OsAlR1 expression was induced by Al stress.Knockout of OsAlR1 affected root development in rice.After Al treatment,compared with the wild type,the osalr1-3 and osalr1-6 lines accumulated more Al³⁺ and ROS,while the OE-OsAlR1-5 and OE-OsAlR1-8 lines accumulated less Al³⁺ and ROS.Knockout of OsAlR1 led to decreased glutathione(GSH)content and superoxide dismutase(SOD)activity in roots.qRT-PCR results revealed that genes regulating the phenylpropanoid biosynthesis pathway(OsPAL7,OsHCT4,OsCCR18,Os02g0467600,PRX82,and Os06g0522300)showed significant expression changes in the knockout lines after Al treatment.[Conclusion]OsAlR1 expression is induced by Al stress,and OsAlR1 alleviates ROS-mediated oxidative stress by increasing GSH content and SOD activity.OsAlR1 may affect Al tolerance in rice by regulating the phenylpropanoid biosynthesis pathway.关键词
水稻/铝胁迫/根系/ROS/抗氧化酶Key words
rice/aluminum stress/roots/ROS/antioxidant enzyme引用本文复制引用
王镜博,苏畅,冯晶,姜思旭,徐海,崔志波,赵明辉..水稻OsAlR1基因耐铝性功能研究[J].中国水稻科学,2025,39(5):615-623,9.基金项目
辽宁省科技重大专项(2022JH1/10200003) （2022JH1/10200003）
国家现代农业产业技术体系建设专项(CARS-01-09). （CARS-01-09）
