中国水稻科学2025，Vol.39Issue(5)：624-634,11.DOI:10.16819/j.1001-7216.2025.240106
基于M1TDS靶向筛选技术的重离子束诱变定向改良杂交水稻卓两优1126性状的研究
Directed Improvement of Hybrid Rice Zhuoliangyou 1126 by Heavy Ion Beam Mutagenesis Based on M1TDS Targeted Screening Technology
摘要
Abstract
[Objective]Using the two-line hybrid rice Zhuoliangyou 1126 as the base variety,we aimed to rapidly develop upgraded varieties with traits such as low cadmium accumulation,fragrance,storage tolerance,submergence tolerance,and a low glycemic index.[Method]Zhuo 234S and Xiangnonghui 1126,the parental lines of Zhuoliangyou 1126,were mutagenized using heavy-ion beams.Chimeric mutants of the OsNRAMP5,OsBADH2,OsLOX3,OsPAO5,OsSSIIIa,and OsBEIIb genes were identified in the M1 generation via M1TDS technology.Mutations in the target genes were further detected and isolated in the M2 generation using Kompetitive Allele-Specific PCR(KASP)genotyping.Hybrid rice with low cadmium accumulation was developed by crossing parents carrying OsNRAMP5 mutations.[Results]A total of 13 chimeric mutants involving six target genes were identified in the M1 generation.Among these,seven were confirmed to carry homozygous or heterozygous mutations in the M2 generation.The cadmium content in OsNRAMP5 homozygous mutants was significantly lower than in the wild type,while the content of the aromatic compound 2-acetylpyrroline(2-AP)was significantly higher in OsBADH2 homozygous mutants.When the low-cadmium hybrid rice derived from OsNRAMP5-mutated parents was pot-grown in cadmium-contaminated soil(available Cd:0.677 mg/kg,pH 5.6),the cadmium content in grains remained consistently below 0.05 mg/kg,compared to 0.91 mg/kg in the wild-type control.[Conclusion]The combination of heavy-ion beam mutagenesis and M1TDS technology enabled the rapid improvement of traits such as low cadmium accumulation in Zhuoliangyou 1126.This study provides a successful example and a general technical reference for transitioning from traditional mutagenesis breeding to directed mutagenesis breeding.关键词
水稻/重离子束诱变育种/镉低积累/香味Key words
rice/heavy ion beam mutation breeding/low cadmium accumulation/fragrance引用本文复制引用
韶也,李文建,罗武中,罗治斌,袁远涛,李曜魁,张丹,周利斌,柏连阳,唐文帮,赵炳然,胡远艺,彭彦,毛毕刚,刘慧敏,唐婵娟,雷斌,唐丽,余丽霞..基于M1TDS靶向筛选技术的重离子束诱变定向改良杂交水稻卓两优1126性状的研究[J].中国水稻科学,2025,39(5):624-634,11.基金项目
长沙市"揭榜挂帅"重大科技攻关项目(kq2301001) （kq2301001）
现代农业产业技术体系资助项目(CARS-01-01) （CARS-01-01）
湖南省2023年度十大技术攻关项目(2023NK1010). （2023NK1010）
