中国水稻科学2025，Vol.39Issue(5)：635-642,8.DOI:10.16819/j.1001-7216.2025.241114
用于水稻品种真实性验证的SNP位点评价
Evaluation of SNP Loci for Rice Variety Authenticity Verification
摘要
Abstract
[Objective]A highly discriminative combination of SNP loci is crucial for developing efficient,accurate,and cost-effective rice variety verification technologies,which can be widely applied in seed regulation,variety breeding and other fields.[Method]This study established fingerprints for 1,383 inbred and 2,702 hybrid rice varieties using the 96 SNP panel from the agricultural industry standard,designated RGIsnp96.[[Result]The results demonstrated that RGIsnp96 exhibited high polymorphism and strong discriminative power for variety identification.For 1,387 inbred varieties,the heterozygous rate,average polymorphic information content(PIC),and average minor allele frequency(MAF)of SNPs in RGIsnp96 were 0.03,0.30,and 0.27,respectively.For 2702 hybrid varieties,the corresponding values were 0.39,0.30,and 0.29,respectively.The identification rates,represented by the proportion of distinguishable variety pairs,for inbred and hybrid rice varieties were 99.94%and 99.99%,respectively.A good equivalence between RGIsnp96 and 48 SSRs in agricultural industry standards(designated RGIssr48 in the following)was evaluated by comparing similarities of variety pairs measured by the two marker sets for both inbred and hybrid varieties.Furthermore,combining RGIsnp96 and RGIssr48,representing different types of markers,could generate better results than using a single type of marker for differentiating certain inbred rice varieties,and corresponding strategies were recommended.[Conclusion]RGIsnp96 demonstrates exceptional capability in rice variety identification,providing robust technical support for improving rice seed quality,regulating the seed market order,and safeguarding farmers'rights and interests.关键词
水稻品种/真实性鉴定/RGIsnp96/单核苷酸多态性/品种鉴定Key words
rice variety/allthenticity verification/RGIsnp96/single nucleotide polymorphism/variety identification引用本文复制引用
徐群,孙燕飞,杨窑龙,魏兴华,王珊,袁筱萍,金石桥,晋芳,郝万军,吴小碧,冯跃,余汉勇..用于水稻品种真实性验证的SNP位点评价[J].中国水稻科学,2025,39(5):635-642,8.基金项目
农业生物育种重大专项子课题资助项目(2022ZD040190202). （2022ZD040190202）
