中国水稻科学2025，Vol.39Issue(5)：643-649,7.DOI:10.16819/j.1001-7216.2025.240501
利用CRISPR/Cas9技术创制无芽鞘紫线的香型环境非敏感隐性核雄性不育种质
Creating a Fragrant Environment-insensitive Recessive Genic Male Sterile Germplasm Lacking Coleoptile Purple Lines by CRISPR/Cas9 Gene Editing
摘要
Abstract
[Objective]Hybrid rice has developed into the third generation in which environment-insensitive recessive genic male sterility(EI-RGMS)is the essential trait.In many rice breeding programs,fragrance improvement is an important target.Additionally,the purple line in coleoptile is a clearly identifiable anthocyanin trait at the very early germination stage,which facilitates simple,rapid,and accurate identification of hybrid purity.The purpose of this study is to develop a fragrant EI-RGMS material lacking the coleoptile purple line based on maintainer II-32B,providing a new core germplasm for the third-generation hybrid rice.[Methods]Using CRISPR/Cas9,we knocked out the pollen development gene OsbHLH141,fragrance gene OsBadh2,and coleoptile purple line gene OsMYB76 in the elite rice parent II-32B.[Results]Mutant lines with all three genes knocked out showed loss of anthocyanin traits,a distinct aroma,and male sterility.[Conclusion]This study successfully created a fragrant EI-RGMS germplasm without purple line in coleoptile in the II-32B background.关键词
环境非敏感/隐性核雄性不育/香味/芽鞘紫线/CRISPR/Cas9Key words
environment-insensitive/recessive genic male sterility/fragrance/purple line in coleoptile/CRISPR/Cas9引用本文复制引用
杨佳欣,张毅,管玉圣,杜润,李贤勇,蔡座坤,王楚桃,阳启样,何永歆,朱子超..利用CRISPR/Cas9技术创制无芽鞘紫线的香型环境非敏感隐性核雄性不育种质[J].中国水稻科学,2025,39(5):643-649,7.基金项目
重庆市农业科学院市级财政科研重点基础研究项目(cqaas2023sjczzd006) （cqaas2023sjczzd006）
重庆市技术创新与应用发展专项重点项目(CSTB2022TIAD-KPX0014). （CSTB2022TIAD-KPX0014）
