中国医学科学院学报2025，Vol.47Issue(4)：611-620,10.DOI:10.3881/j.issn.1000-503X.16367
胆道类器官的构建及应用进展
Advances in Construction and Application of Biliary Organoids
摘要
Abstract
Biliary duct injury,congenital biliary atresia,biliary tract tumors,primary sclerosing cholangitis,etc.are common and refractory diseases in the digestive system in clinical practice.The existing sur-gical operations and drug treatments demonstrate limited effects.Organoids,as an emerging technology,have at-tracted much attention in recent years for deeply understanding the pathogenesis and development of these diseases and seeking more effective treatment approaches.An organoid,a three-dimensional complex derived from stem/progenitor cells,can simulate the complex structure and physiological function of tissues or organs in vitro.It pro-vides an important platform for studying the pathogenesis of biliary tract diseases and brings new hope for the re-pair and regeneration of biliary tract injury.The seed cells for constructing biliary organoids are mainly biliary tract epithelial cells,pluripotent stem cells,etc.The conventional technologies for constructing biliary organoids mainly include embedding,rotary culture,and hanging drop culture.In recent years,new culture technologies such as organ chip and three-dimensional and four-dimensional printing are emerging.This article reviews the con-struction methods of biliary organoids,discusses the application of these organoids in disease model construction,disease mechanism research,drug screening,and tissue/organ repair,and proposes the current problems and future research directions of biliary organoids,which will provide reference for treating common refractory diges-tive system diseases in clinical practice.关键词
类器官/胆道疾病/药物筛选/再生医学Key words
organoid/biliary tract disease/drug screening/regenerative medicine分类
医药卫生引用本文复制引用
雷忠文,向杨,杨毅军..胆道类器官的构建及应用进展[J].中国医学科学院学报,2025,47(4):611-620,10.基金项目
国家自然科学基金(82260136)、海南省重点研发项目(ZDYF2021SHFZ053)、海南省院士创新平台科研专项(YSPTZX202027)和海口市重点科技项目(2022-032) （82260136）
