中国癌症防治杂志2025，Vol.17Issue(4)：408-415,8.DOI:10.3969/j.issn.1674-5671.2025.04.02
碳基负载钌纳米颗粒协同光热-化学动力学疗法抑制乳腺癌细胞增殖与迁移
Ru nanoparticles-carbon support synergize photothermal-chemodynamic therapy to inhibit the proliferation and migration of breast cancer cells
摘要
Abstract
Objective To investigate the effects and underlying mechanisms of Ru nanoparticles-carbon support(Ru-NPs/C)in mediated chemodynamic therapy(CDT)and photothermal therapy(PTT)on the proliferation and migration of breast cancer cells.Methods The Ru-NPs/C were synthesized via a template-sacrificed assisted pyrolysis method,and their structural and catalytic properties were characterized by X-ray diffraction(XRD),transmission electron microscopy(TEM),X-ray photoelectron spectroscopy(XPS),ultraviolet-visible spectrophotometry,and infrared thermography.The effects of Ru-NPs/C,both independently and in conjunction with near-infrared(NIR)radiation,on the proliferation and migration of 4T1 cells were assessed using the MTT assay and scratch test.The biocompatibility was evaluated via a hemolysis assay,and intracellular reactive oxygen species(ROS)level was measured using the 2',7'-DCFH-DA fluorescent probe.Results Ru-NPs/C was successfully synthesized with Ru nanoparticles were highly dispersed on the carbon support.XPS analysis further revealed the co-existence of Ru in mixed valence states(Ru0 and Ru3+).Ru-NPs/C simultaneously mimicked robust peroxidase(POD)-like,catalase(CAT)-like,and glutathione oxidase(GSH-OXD)-like catalytic activities while exhibiting significant photothermal responsiveness.Compared to control and individual treatment groups,the combination of Ru-NPs/C with NIR group markedly increased ROS levels and inhibited the proliferation and migration of 4T1 cells(all P＜0.01).Meanwhile,Ru-NPs/C demon-strated negligible cytotoxicity to normal cells MCF-10A,with a hemolysis rate below 5％even at 150 μg/mL,exhibiting excellent he-mocompatibility.Conclusions Ru-NPs/C possess efficient multi-enzyme catalytic properties.Under NIR stimulation,effectively gener-ate ROS to inhibit the proliferation and migration of breast cancer cells,demonstrating excellent potential for synergistic therapy.关键词
乳腺癌/钌基纳米颗粒/化学动力学疗法/近红外光热治疗/活性氧Key words
Breast cancer/Ruthenium-based nanoparticles/Chemodynamic therapy/Near-infrared photothermal therapy/Reactive oxygen species分类
医药卫生引用本文复制引用
梁可欣,梁祝晓,张维青..碳基负载钌纳米颗粒协同光热-化学动力学疗法抑制乳腺癌细胞增殖与迁移[J].中国癌症防治杂志,2025,17(4):408-415,8.基金项目
广西科技计划项目(桂科AD22035044) （桂科AD22035044）
广西医科大学高水平创新团队及杏湖学者计划 （）
广西医科大学附属肿瘤医院引进高层次人才 （）
