摘要
Abstract
Objective To explore the effect of the interaction between intestinal flora and neurophysiological factors on language development in children with autism spectrum disorder(ASD),and to reveal the potential"gut-brain axis"regulation mechanism.Methods A retrospective cohort study was designed to include 82 children aged 3-8 years with ASD,who were divided into language retardation group(n=52)and normal language development group(n=30)according to Clinical Evaluation of Language Fundamentals-Fifth Edition(CELF-5).The diversity and species composition of intestinal flora were analyzed by 16S rRNA sequencing.The functional connectivity of language network was evaluated by resting state fMRI and EEG spectrum,and 5-Hydroxytryptamine(5-HT).Brain-Derived Neurotrophic Factor(BDNF)and Short-Chain Fatty Acids(SCFAs)were detected by Enzyme-Linked Immunosorbent Assay(ELISA).Results The language development delay group showed a decreased alpha diversity of the gut microbiota(Shannon index:)3.82±0.51 vs 4.25±0.63,P=0.001),Firmicutes(LDA=4.32,P=0.002)and Clostridium perfringens(abundance increased by 3.1 times)were significantly enriched.In the normal language development group,Bacteroidales(LDA=3.78,P=0.008)and Prevotella(LDA=3.92,P=0.004)were dominant.At the neurophysiological level,the functional connectivity intensity of the γ band in the language development delay group decreased by 29.3%(0.29±0.09 vs.0.41±0.11,P<0.001),and the serum 5-HT level increased by 25.3%(182.62±35.42 vs.145.86±28.71 ng/mL)(P<0.001),the levels of BDNF and SCFAs decreased by 23.4%and 16.0%respectively(P<0.05).Conclusion TASD language development delay is associated with intestinal microbiota(Clostridium enrichment),neurotransmitters(5-HT/BDNF imbalance),and neurophysiological characteristics(impaired γ band oscillations).Among them,Prevotella may enhance the functional connectivity of the language network through the SCFas-vagus nerve pathway,although cross-system abnormalities suggest a potential association with the"gut-brain axis".However,the direct cascading causal relationship among the three remains unclear.关键词
孤独症谱系障碍/肠道菌群/神经生理/语言发育/γ频段振荡Key words
Autism spectrum disorder/Intestinal flora/Neurophysiology/Language development/Gamma-band oscillation分类
社会科学
