植物保护学报2025，Vol.52Issue(4)：822-831,10.DOI:10.13802/j.cnki.zwbhxb.2025.2025010
小麦隐性抗白粉病基因pmCH1853的遗传分析与分子定位
Genetic analysis and molecular mapping of a recessive powdery mildew resistance gene pmCH1853 in wheat
摘要
Abstract
To screen for new powdery mildew resistance genes in wheat,phenotypic identification and genetic analysis were conducted on the genetic population derived from the resistant parent CH1853 and susceptible parent Taichang 29.The bulked segregant analysis and 90K single nucleotide polymor-phism(SNP)chip technology were employed for genetic mapping of the powdery mildew resistance gene in the resistant parent CH1853.The results showed that the F1 plants derived from the resistant parent CH1853 and susceptible parent Taichang29 were susceptible to Blumeria graminis f.sp.tritici isolate E09,and their F2 individuals showed segregation of resistance.The ratio of resistant individuals to susceptible individuals roughly followed a 1∶3 segregation ratio in F2 population,indicating that the powdery mildew resistance was controlled by a recessive gene,temporarily named as pmCH1853.A total of 89 SNPs exhibited polymorphism between the resistance bulk and susceptible bulk,of which 44 SNPs were located on chromosome 2D,and 42 SNPs were distributed within the 635.6-650.3 Mb genomic interval.Five simple sequence repeat markers linked to resistance were obtained within this region.Eventually,pmCH1853 was mapped between markers Sxau2DL-115 and Sxau2DL-147,with genetic distances of 0.8 cM and 3.2 cM,respectively.The physical location was narrowed down to the 635.6-639.9 Mb interval.This gene can be used as a novel genetic resource for powdery mildew resis-tance in wheat breeding programs.关键词
小麦/隐性基因/抗白粉病基因/遗传分析/分子定位/遗传距离Key words
wheat/recessive gene/powdery mildew resistance gene/genetic analysis/molecular map-ping/genetic distance引用本文复制引用
陈芳,常松鹤,王聪雅,徐业萍,乔麟轶,李欣,郭宝德,张晓军..小麦隐性抗白粉病基因pmCH1853的遗传分析与分子定位[J].植物保护学报,2025,52(4):822-831,10.基金项目
山西省青年科学研究项目(20210302124234),山西农业大学博士科研启动项目(2021BQ42),山西省农业科学院作物科学研究所博士基金项目(ZB1103),山西省博士毕业生来晋工作奖励资金科研项目(SXBYKY2021051) （20210302124234）
