植物保护学报2025，Vol.52Issue(4)：832-841,10.DOI:10.13802/j.cnki.zwbhxb.2025.2025008
甲基二磺隆胁迫下抗、感小麦品种的生理响应差异
Differences in physiological responses of resistant and susceptible wheat varieties to mesosulfuron-methyl stress
摘要
Abstract
To examine the physiological mechanisms that underlie the observed variations in resistance to herbicide metildisulfuron among diverse wheat varieties,the effects of mesosulfuron-methyl on the growth of seedlings of the resistant wheat variety Chang 4640(C-4640)and the susceptible variety Henong 4198(H-4198)were determined,along with the response differences in antioxidant enzyme activities,malondialdehyde(MDA)content,chlorophyll content,and photosynthetic characteristic parameters under 15.75 g(a.i.)/hm2 mesosulfuron-methyl stress(the maximum recommended field dose).Correlation analysis and principal component analysis(PCA)were further employed to identify key indicators for evaluating herbicide resistance in wheat varieties.The study showed that the EC50 values of mesosulfuron-methyl for C-4640 and H-4198 were 63.20 g(a.i.)/hm2 and 4.22 g(a.i.)/hm2,respectively,with the former being approximately 15 times higher than the latter.Under 15.75 g(a.i.)/hm2 mesosulfuron-methyl stress,the activities of superoxide dismutase,peroxidase,and catalase in both C-4640 and H-4198 increased,peaking on day 5 after treatment,and the antioxidant enzyme activities were generally higher in C-4640 than in H-4198;MDA content in C-4640 showed an initial increase fol-lowed by a decrease,while in H-4198,it decreased by 28.62%relative to the control on day 5 before rising continuously;chlorophyll content in H-4198 decreased significantly compared with the control.However,a decline in photosynthetic parameters,including stomatal conductance,transpiration rate,and net photosynthetic rate,was observed in the experimental group when compared with the control group.In contrast,C-4640 exhibited no statistically significant alterations in these parameters.The evaluation of wheat resistance to mesosulfuron-methyl can be facilitated by the use of key indicators,including chloroplast pigment content,photosynthetic characteristics,and antioxidant enzyme activity.关键词
小麦/甲基二磺隆/生理响应/抗氧化酶活性/光合参数Key words
wheat/mesosulfuron-methyl/physiological response/antioxidant enzyme activity/photo-synthetic parameter引用本文复制引用
侯燕茹,王贵启,张颖君,刘小民,赵杰,高兴祥,赵铂锤,郭兵博,许贤,李秉华,谷旭,魏帅坤..甲基二磺隆胁迫下抗、感小麦品种的生理响应差异[J].植物保护学报,2025,52(4):832-841,10.基金项目
国家重点研发计划(2023YFD1400500),河北省农林科学院科技创新专项课题(2022KJCXZX-LYS-13),河北省农林科学院粮油作物研究所青年创新基金(2024LYS05) （2023YFD1400500）
