植物保护学报2025，Vol.52Issue(4)：842-851,10.DOI:10.13802/j.cnki.zwbhxb.2025.2025064
黄瓜枯萎病生防菌株的筛选鉴定及防治效果
Screening,identification and control efficacy of biocontrol strains for controlling cucumber Fusarium wilt
摘要
Abstract
To screen biocontrol microbial resources for controlling cucumber Fusarium wilt,the plate confrontation method was adopted to screen antagonistic strains against Fusarium oxysporum f.sp.cuc-umerinum.Morphological,physiological and biochemical,and molecular biology identification were performed.The effects of these antageonistic strain on spore germination of F.oxysporum f.sp.cuc-umerinum and cucumber seed germination,antibacterial spectrum,and extracellular hydrolase produc-tion characteristics were determined.Additionally,the biocontrol effect was evaluated through pot and field experiments.The results showed that the strain Sneb2570 with strong antagonistic activity against F.oxysporum f.sp.cucumerinum was screened from 88 bacteria maintained in our laboratory,with an inhibition rate of 61.64%.Strain Sneb2570 was ultimately identified as Bacillus velezensis.This strain significantly inhibited spore germination of F.oxysporum f.sp.cucumerinum.It did not affect the germi-nation of cucumber seeds,but promoted the growth of radicles.Simultaneously,it exhibited inhibitory effects against six other plant pathogenic fungi.Additionally,the strain Sneb2570 was found to produce protease,phosphatase,and amylase.Root irrigation with the fermentation broth of strain Sneb2570 pro-moted the growth of cucumber seedlings.The control effects of Sneb2570 fermentation broth against cucumber Fusarium wilt disease in potted and field treatments were 44.12%and 45.24%,respectively.Collectively,strain Sneb2570 can be further developed and utilized as a promising biocontrol agent for controlling cucumber Fusarium wilt.关键词
贝莱斯芽胞杆菌/黄瓜枯萎病/鉴定/防效/种子萌发/生长Key words
Bacillus velezensis/cucumber Fusarium wilt/identification/control efficacy/seed germina-tion/growth引用本文复制引用
黄馨玉,朱晓峰,王媛媛,刘晓宇,赵迪,杨宁,段玉玺,范海燕,陈立杰..黄瓜枯萎病生防菌株的筛选鉴定及防治效果[J].植物保护学报,2025,52(4):842-851,10.基金项目
中国博士后科学基金特别资助(站中)项目(2022T150442),辽宁省自然科学基金面上项目(2024-MS-091),中国博士后科学基金(2021M692234) （站中）
