中药与临床2025，Vol.16Issue(5)：37-42,6.
一测多评法同时测定创伤消肿片中7种皂苷成分含量
Determination of seven saponins in Chuangshangxiaozhong Tablets with quantitative analysis of multi-components by single marker
摘要
Abstract
Objective:To develop a quantitative analysis method using single marker for multi-component determination(QAMS)to simultaneously assess the content of seven saponins in Chuangshangxiaozhong Tablets,and subsequently evaluate the adaptability and effectiveness of the QAMS method in the quality control of Chuangshangxiaozhong Tablets.Methods:By employing the HPLC tech-nique and utilizing ginsenoside Rg1 as the internal standard,the relative factors(fs/i)for notoginsenoside R1,ginsenoside Re,ginsenoside Rb1,ginsenoside Ro,chikusetsusaponin Ⅳ and chikusetsusaponin Ⅳa were successfully determined.These factors were then used as the foundation for accurately calculating the contents of seven saponins in Chuangshangxiaozhong Tablets.In parallel,the external standard method(ESM)was also utilized to establish a benchmark for saponin content.Then,a comparative analysis of the results obtained from QAMS and ESM were compared.Results:fs/i of notoginsenoside R1,ginsenoside Re,ginsenoside Rb1,ginsenoside Ro,chikusetsusapo-nin Ⅳ,and chikusetsusaponin Ⅳa were 1.293 7,1.138 2,1.215 3,1.081 3,0.912 2 and 0.804 8,respectively.After comparative analysis,it was found that when using both the QAMS and ESM method to determine the content of components in 10 batches of trauma detu-mescence tablets,the results obtained by the two methods showed a high degree of consistency,and there was no significant difference in the content of each component.Conclusion:QAMS demonstrates equivalence to ESM in evaluating the component content of Chuang-shangxiaozhong Tablets,indicating its potential as a robust tool for quantitative analysis and quality assessment of saponins in this prepa-ration.关键词
创伤消肿片/一测多评/相对校正因子/人参皂苷Rg1Key words
ChuangshangxiaozhongTablets/quantitative analysis of multi-components by single marker/relative calibrationfac-tor/ginsenoside Rg1分类
医药卫生引用本文复制引用
刘李梅,李敏敏,彭伟,吴明权,涂禾..一测多评法同时测定创伤消肿片中7种皂苷成分含量[J].中药与临床,2025,16(5):37-42,6.基金项目
四川省自然科学基金青年科学基金项目(2024NSFSC1853) （2024NSFSC1853）
