中国免疫学杂志2025，Vol.41Issue(9)：2061-2066,6.DOI:10.3969/j.issn.1000-484X.2025.09.003
基于PI3K-AKT/NF-κB信号通路探讨芡实合剂对狼疮性肾炎小鼠肾损伤的影响
Effects of semen euryales mixture on kidney injury in lupus nephritis mice based on PI3K-AKT/NF-κB signaling pathway
摘要
Abstract
Objective:To explore the effect and possible mechanism of Semen euryales mixture(QSHJ)on renal injury in lu-pus nephritis.Methods:The 14-week-old C57BL/6 mice were the control group of 8.The 14-week-old MRL/lpr mice were randomly di-vided into model group,QSHJ low,medium and high doses groups(7.48,14.95,29.90 g/kg),and prednisone group(5 mg/kg)of 8 mice each.The control group and the model group were given the same volume of normal saline by gavage once a day for 4 weeks.Urine was collected to detect urinary protein content.HE and PASM staining were used to observe renal tissue injury.The serum levels of Anti-dsDNA,IL-6 and TNF-α were detected by ELISA.The protein expressions of phosphatidylinositol 3-kinase(PI3K),phosphor-ylated PI3K(p-PI3K),protein kinase B(AKT),phosphorylated AKT(p-AKT),nuclear factor kappa B p65(NF-κB p65)and phos-phorylated NF-κB p65(p-NF-κB p65)were detected by Western blot.The protein expressions of PI3K,p-PI3K,AKT and p-AKT were detected by immunohistochemistry.The positive expression of NF-κB p65 was observed by immunofluorescence.Results:Com-pared with the control group,the urinary protein content in the model group increased significantly(P<0.05),the renal tissue was in-filtrated with inflammatory cells,and the tissue structure was destroyed obviously,the contents of Anti-dsDNA,IL-6 and TNF-α in se-rum were significantly increased(P<0.05),the expressions of p-PI3K/PI3K,p-AKT/AKT and p-NF-κB p65/NF-κB p65 were signifi-cantly increased(P<0.05),the protein expressions of p-PI3K and p-AKT were increased significantly(P<0.05),the positive expres-sion of NF-κB p65 was increased significantly.Compared with the model group,the urinary protein content of QSHJ low,medium and high doses groups and prednisone group decreased significantly(P<0.05),the infiltration of inflammatory cells in renal tissue was re-lieved,and the damage of tissue structure was alleviated.the contents of Anti-dsDNA,IL-6 and TNF-α in serum were significantly de-creased(P<0.05),the expressions of p-PI3K/PI3K,p-AKT/AKT and p-NF-κB p65/NF-κB p65 were significantly decreased(P<0.05),the protein expressions of p-PI3K and p-AKT were significantly decreased(P<0.05),the positive expression of NF-κB p65 flu-orescence was decreased.Conclusion:QSHJ can improve the pathological manifestations of renal tissue in MRL/lpr mice and alleviate the disease process.The mechanism may be related to the inhibition of PI3K-AKT/NF-κB signaling pathway in renal tissue and the re-duction of inflammatory response by QSHJ.关键词
系统性红斑狼疮/狼疮性肾炎/芡实合剂/PI3K-AKT/NF-κB信号通路Key words
Systemic lupus erythematosus/Lupus nephritis/Semen euryales mixture/PI3K-AKT/NF-κB signaling pathway分类
医药卫生引用本文复制引用
陈一鸣,武上雯,邢清桦,李桓,李松伟..基于PI3K-AKT/NF-κB信号通路探讨芡实合剂对狼疮性肾炎小鼠肾损伤的影响[J].中国免疫学杂志,2025,41(9):2061-2066,6.基金项目
国家自然科学基金面上项目(81874465) （81874465）
河南省中医药科学研究专项(20-21ZYZD16). （20-21ZYZD16）
