中医药学报2025，Vol.53Issue(9)：10-16,7.DOI:10.19664/j.cnki.1002-2392.250180
基于SOD/Nrf2/HO-1信号通路探讨温和汤调节热性惊厥大鼠铁死亡的药效机制
Mechanism of Wenhe Decoction in Regulating Ferroptosis in Febrile Seizure Rats via SOD/Nrf2/HO-1 Signaling Pathway
摘要
Abstract
Objective:To investigate the regulatory mechanism of Wenhe Decoction on ferroptosis in hot water bath-induced febrile seizure(FS)model rats through SOD/Nrf2/HO-1 signaling pathway,thereby restoring Glu/GABA balance.Methods:Fifty SD rats were randomly divided into blank group,model group,and low-,medium-,high-dose Wenhe Decoction groups(10 rats each).After successful modeling,the three Wenhe Decoction groups received 4.05 g/kg,8.1 g/kg,and 16.2 g/kg(1∶2∶4 ratio)respectively via gavage(10 mL/kg);blank and model groups received equal-volume distilled water,once daily for21 days.Except blank group,rats were placed in(45±0.5)℃water bath until seizure onset,then immediately removed and dried;control group was placed in(37±0.5)℃bath for 5 minutes.Seizure latency,duration,and behavioral symptoms were recorded.Hippocampal pathology was observed via HE and Nissl staining.ROS levels were detected by DHE fluorescence probe.Serum iron,ATP,Glu,GABA,SOD,Nrf2,and HO-1 were measured by ELISA.Results:Compared with blank group,FS model rats showed shortened seizure latency,prolonged duration,increased seizure grade/incidence(P<0.05,P<0.01);hippocampal neurons exhibited loss,reduced count,irregular morphology,and disarrangement;serum iron,ROS,Glu,SOD,Nrf2,and HO-1 significantly increased(P<0.05,P<0.01),while ATP and GABA decreased(P<0.01).Compared with model group,all Wenhe Decoction doses improved behavioral symptoms,hippocampal neuron arrangement,and reduced pathological damage;serum iron,ROS,Glu,SOD,Nrf2,and HO-1 decreased(P<0.05,P<0.01),while ATP and GABA increased(P<0.01).Conclusion:Wenhe Decoction may activate SOD/Nrf2/HO-1 pathway to inhibit ferroptosis,restore Glu/GABA balance,reduce neuronal damage and FS recurrence.关键词
热性惊厥/铁死亡/温和汤/SOD/Nrf2/HO-1信号通路Key words
Febrile seizure/Ferroptosis/Wenhe Decoction/SOD/Nrf2/HO-1 signaling pathway分类
医药卫生引用本文复制引用
余欢,邓羿駃,李鑫,王孟清,帅云飞..基于SOD/Nrf2/HO-1信号通路探讨温和汤调节热性惊厥大鼠铁死亡的药效机制[J].中医药学报,2025,53(9):10-16,7.基金项目
国家自然科学基金项目(82174437) （82174437）
湖南省教育厅资助科研项目(19B425) （19B425）
湖南中医药大学中医儿科学湖南省"双一流"学科建设项目(4912-0005001010) （4912-0005001010）
