中医药学报2025，Vol.53Issue(9)：39-44,6.DOI:10.19664/j.cnki.1002-2392.250184
复合酶法提取太白贝母中多糖工艺及其抗氧化活性研究
Study on the Polysaccharide Extraction Process from Fritillaria taipaiensis Using a Compound Enzyme Method and Its Antioxidant Activity
摘要
Abstract
Objective:To optimize the extraction process of polysaccharides from Fritillaria taipaiensis using a compound enzyme method and to explore its antioxidant activity.Methods:Based on preliminary experiments with a fixed solid-liquid ratio of 1∶15 g/mL,the extraction process was optimized using response surface methodology.The independent variables were the amount of compound enzyme,enzymatic hydrolysis time,enzymatic hydrolysis temperature,and solution pH,while the dependent variable was the polysaccharide extraction rate.The antioxidant activity of the extracted polysaccharides was evaluated by measuring their DPPH free radical scavenging ability and Fe3+reduction capacity.Results:The optimal extraction conditions were as follows:compound enzyme amount 2.3%,enzymatic hydrolysis time 63 minutes,pH 5.2,and enzymatic hydrolysis temperature 43℃.Under these conditions,the actual extraction rate of polysaccharides was 10.034 9%,which was very close to the predicted value of 10.050 1%,with a difference of only 0.015 2%,indicating that the model was reliable and could be used for predicting polysaccharide extraction.Antioxidant experiments showed that at a high concentration of 3.0 mg/mL,Fritillaria taipaiensis polysaccharides exhibited DPPH radical scavenging and Fe3+reducing abilities similar to those of the positive control(vitamin C)at the same concentration,with no significant differences.Conclusion:Fritillaria taipaiensis polysaccharides possess significant antioxidant activity,providing experimental evidence for the development and application of this medicinal herb.This study also offers new insights and methods for the application of polysaccharides in the fields of health food and medicine.关键词
太白贝母/多糖/复合酶/提取工艺/抗氧化活性Key words
Fritillaria taipaiensis/Polysaccharides/Compound enzyme/Extraction process/Antioxidant activity分类
医药卫生引用本文复制引用
侯敏娜,侯少平,王洁,彭修娟,许海燕,赵帆..复合酶法提取太白贝母中多糖工艺及其抗氧化活性研究[J].中医药学报,2025,53(9):39-44,6.基金项目
陕西省中药质量标志物创新团队(SSY18TD02) （SSY18TD02）
2022年陕西省教育厅重点科研计划项目(协同创新中心项目22JY005) （协同创新中心项目22JY005）
