安徽医科大学学报2025，Vol.60Issue(8)：1373-1380,8.DOI:10.19405/j.cnki.issn1000-1492.2025.08.002
网络药理学视角下山奈酚靶向BCL-2抑制肺癌的机制研究
Research on the mechanism of Kaempferol targeting BCL-2 to inhibit lung cancer from the perspective of network pharmacology
摘要
Abstract
Objective To investigate the potential therapeutic mechanisms of kaempferol,an active component in the traditional Chinese medicine gardenia,for lung cancer treatment using a network pharmacology approach.Methods The main active ingredients and potential targets of Gardenia jasminoides were obtained through the Tra-ditional Chinese Medicine Pharmacology Database and Analysis Platform(TCMSP),and combined with the lung cancer related target information collected from Gene Cards and OMIM databases,the intersection targets of Garde-nia jasminoides and lung cancer treatment were determined by drawing Venn diagrams.Further screening of core targets was conducted through PPI network analysis,and gene ontology(GO)function and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed using the Metascape platform.Auto dock software was used to evaluate the binding affinity between the active ingredients of Gardenia jasminoides and target proteins.In terms of experiments,cell proliferation ability was evaluated through CCK-8 assay,cell migration and invasion ability were detected through cell scratch healing assay and Transwell assay,and the expression levels of epithelial mesenchymal transition(EMT)protein and inflammatory factors were detected by Western blot and RT-qPCR.Results The active ingredient kaempferol in Gardenia jasminoides exhibited significant binding ability to the key target of lung cancer,B-cell lymphoma-2(BCL-2),and could inhibit the proliferation,migration,and invasion of lung cancer cells.The results of Western blot and RT-qPCR further confirmed that kaempferol could promote an increase in E-cadherin,a decrease in N-cadherin and Vimentin,and reduce the expression of inflam-matory factors.Conclusion The active ingredient of Gardenia jasminoides,kaempferol,inhibits the proliferation,migration and invasion of lung cancer cells by targeting BCL-2,while reversing EMT progression and suppressing the expression levels of inflammatory cytokines in lung cancer cells,thus preventing lung cancer progression.关键词
栀子/山奈酚/肺癌/BCL-2/网络药理学Key words
gardenia jasminoides/kaempferol/lung cancer/BCL-2/network pharmacology分类
医药卫生引用本文复制引用
陈建栋,吕盈盈,徐正,张苗,刘路遥,王鹏..网络药理学视角下山奈酚靶向BCL-2抑制肺癌的机制研究[J].安徽医科大学学报,2025,60(8):1373-1380,8.基金项目
国家自然科学基金项目(编号:82204845) （编号:82204845）
上海市自然科学基金项目(编号:22ZR1447900) National Natural Science Foundation of China(No.82204845) （编号:22ZR1447900）
Natural Science Foundation of Shanghai(No.22ZR1447900) （No.22ZR1447900）
