安徽医科大学学报2025，Vol.60Issue(8)：1381-1386,6.DOI:10.19405/j.cnki.issn1000-1492.2025.08.003
关节内出血对大鼠伸直型膝关节挛缩的影响
Effect of intra-articular hemorrhage on extending knee joint contracture in rats
摘要
Abstract
Objective To investigate the effect of intraarticular hemorrhage on extending knee joint contracture model in rats.Methods 18 mature male SD rats were divided into 3 groups by random number table method.The control group(group C)was not immobilized and was killed after 4 weeks of feeding.In the simple fixation group(M1 group),the left lower limb knee joint was immobilized in straight position for 4 weeks.The blood fixation group(M2 group)was injected into the knee cavity with body blood and immobilized in a straight position for 4 weeks.The knee joint motion of each group was measured by the joint motion measuring instrument under a stand-ard torque.The contracture degree was calculated by the joint range of motion of the knee joint before and after muscles separation.HE staining and Masson staining were used to detect the number of cells and collagen deposi-tion in the anterior joint capsule.The protein expressions of transforming growth factor 1(TGF-β1),wingless-type MMTV integration site family,member 1(Wnt1)and beta-catenin(β-catenin)in the anterior articular capsule were detected by Western blotting.Results Compared with group C,total knee contracture and arthrogenic con-tracture of rats in M1 and M2 groups increased,and the difference was statistically significant(P＜0.05).At the same time,the degree of total contracture and arthrogenic contracture in M2 group was higher than that in M1 group,and the difference was statistically significant(P＜0.05).Compared with group C,the number of anterior joint capsule cells and collagen deposition in M1 and M2 groups increased,and the difference was statistically sig-nificant(P＜0.05).At the same time,the number of anterior joint capsule cells and collagen deposition in M2 group were higher than those in M1 group,and the difference was statistically significant(P＜0.05).Compared with group C,the protein expressions of TGF-β1,Wnt1 and β-catenin in the anterior articular capsule of rats in M1 and M2 groups increased,and the difference was statistically significant(P＜0.05).At the same time,the protein expressions of TGF-β1,Wnt1 and β-catenin in the anterior articular capsule of the knee joint in M2 group were sig-nificantly higher than those in M1 group,with statistical significance(P＜0.05).Conclusion Joint immobiliza-tion can lead to joint contracture,and joint bleeding aggravates the degree of joint capsule fibrosis induced by im-mobilization.关键词
膝关节挛缩/纤维化/膝关节活动度/关节内出血/Wnt/β-catenin信号通路Key words
knee joint contracture/fibrosis/knee joint range of motion/joint hemorrhage/Wnt/β-catenin signa-ling pathway分类
医药卫生引用本文复制引用
张全兵,张润,朱得婷,周云,阚秀丽,霍雷,张若希,肖晗,毛晶,李雪明..关节内出血对大鼠伸直型膝关节挛缩的影响[J].安徽医科大学学报,2025,60(8):1381-1386,6.基金项目
安徽省自然科学基金项目(编号:2408085QH270) （编号:2408085QH270）
安徽省卫生健康科研项目(编号:AHWJ2022b063、AHWJ2023A30077) （编号:AHWJ2022b063、AHWJ2023A30077）
安徽省高等学校省级质量工程项目(编号:2023jyxm1129) （编号:2023jyxm1129）
安徽医科大学第二附属医院国自然孵育计划项目(编号:2022GMFY05) Natural Science Foundation of Anhui Province(No.2408085QH270) （编号:2022GMFY05）
Health Research Project of Anhui Province(Nos.AHWJ2022b063,AHWJ2023A30077) （Nos.AHWJ2022b063,AHWJ2023A30077）
Provincial Quality Project of Colleges and Uni-versities in Anhui Province(No.2023jyxm1129) （No.2023jyxm1129）
National Natural Science Foundation Incubation Plan of the Sec-ond Affiliated Hospital of Anhui Medical University(No.2022GMFY05) （No.2022GMFY05）
