安徽医科大学学报2025，Vol.60Issue(8)：1393-1400,8.DOI:10.19405/j.cnki.issn1000-1492.2025.08.005
DHX32在胰腺癌中的功能、临床意义及预后价值
The cell biological function,clinical significance and prognosis values of DHX32 in pancreatic cancer
摘要
Abstract
Objective To study the expression and prognosis of DHX32 in pancreatic cancer and observe the bio-logical function of DHX32 in pancreatic cancer cell line SW1990.To investigate the clinical significance and prog-nostic value of DHX32 in pancreatic cancer,and predict the possible related mechanism.Methods GEPIA data-base was used to analyze the expression and prognosis of DHX32 in pancreatic cancer,and TCGA database was used to analyze the relationship between DHX32 mRNA expression level and clinicopathological features and prog-nosis of patients with pancreatic cancer.The effects of DHX32 on the proliferation,invasion and migration of SW1990 pancreatic cancer cells were studied by EdU immunofluorescence assay,Transwell assay and scratch as-say.GSEA was used to predict DHX32 the possible related signaling pathways involved with DHX32 in pancreatic cancer.Results The results of GEPIA database analysis showed that the expression level of DHX32 in pancreatic cancer tumor tissues was significantly higher than that in normal pancreatic tissues(P＜0.05).Meanwhile,the prognosis of pancreatic cancer patients with high DHX32 expression was worse than that of patients with low DHX32 expression(P＜0.001).Cox regression analysis showed that DHX32 mRNA expression level and M stage were in-dependent risk factors for prognosis of pancreatic cancer patients(all P＜0.05).EdU immunofluorescence assay,Transwell assay and scratch assay confirmed that the overexpression of DHX32 promoted the proliferation,invasion and migration of pancreatic cancer cells(all P＜0.001),while silencing DHX32 inhibited the proliferation,inva-sion and migration of pancreatic cancer cells(all P＜0.001).The results of GSEA enrichment analysis showed that DHX32 was enriched in four signaling pathways:hematopoietic cell lineage,neuroactive ligand receptor inter-action,primary immune deficiency and spliceosome(all P＜0.05).Conclusion DHX32 is highly expressed in pancreatic cancer.It promotes the proliferation,invasion and metastasis of pancreatic cancer cells,and is closely related to the poor prognosis of pancreatic cancer patients.关键词
DHX32/胰腺癌/功能/临床意义/预后Key words
DHX32/pancreatic carcinoma/function/clinical significance/prognosis分类
医药卫生引用本文复制引用
马丹丹,张钦泉,董毅,李中虎,张智勇..DHX32在胰腺癌中的功能、临床意义及预后价值[J].安徽医科大学学报,2025,60(8):1393-1400,8.基金项目
国家自然科学基金项目(编号:81902501) （编号:81902501）
湖北省自然科学基金项目(编号:2022CFD112) National Natural Science Foundation of China(No.81902501) （编号:2022CFD112）
Natural Science Foundation of Hubei Province(No.2022CFD112) （No.2022CFD112）
