安徽医科大学学报2025，Vol.60Issue(8)：1401-1409,9.DOI:10.19405/j.cnki.issn1000-1492.2025.08.006
硝酸盐通过调控EGFR-AKT/ERK1/2信号通路预防咬肌萎缩
Prophylactic nitrate supplementation mediates EGFR-AKT/ERK1/2 pathway to prevent masseter muscle atrophy
摘要
Abstract
Objective To investigate the effects of occlusal alterations on masseter muscle atrophy after the applica-tion of excessive orthodontic tension,and the role and mechanism of prophylactic nitrate supplementation in the pre-vention and treatment of masseter muscle atrophy.Methods Eight-week-old male SD rats were selected.An ortho-dontic appliance was placed in the oral cavity of the rats to obtain a rat model of occlusal alterations,and the mas-seter muscles were taken at various times after excessive orthodontic force application for relevant tests.Masseter muscle weighing,HE staining,Western blot and immunohistochemistry assays were used to detect the atrophic state of the masseter muscle.Meanwhile,immunofluorescence staining was used to detect the hypoxic status of the mas-seter muscle,and immunofluorescence and immunohistochemical staining were used to detect the angiogenic status[cluster of differentiation 31(CD31),vascular endothelial growth factor(VEGF)].After 28 days of prophylactic nitrate supplementation and application of orthodontic tension,the status of masseter muscle atrophy,tissue hypoxi-a,and angiogenesis were assessed as described above.In addition,Western blot assays for detecting epidermal growth factor receptor(EGFR),protein kinase B(AKT),extracellular signal-regulated kinases 1/2(ERK1/2)protein expressions,and their corresponding phosphorylated proteins.One step TUNEL apoptosis assay kit was used to detect masseter muscle apoptosis.Results The molar displacement caused by overload traction could lead to masseter muscle atrophy,accompanied by hypoxia in the masseter tissue and decreased expression of angiogenesis-related proteins.Preventive nitrate supplementation could prevent masseter muscle atrophy,alleviate tissue hypoxi-a,and promote angiogenesis.The anti-apoptotic effects of the EGFR-AKT/ERK1/2 signaling pathway contributed to the preventive effects of nitrate on masseter muscle atrophy.Conclusion Occlusal alterations brought about by excessive orthodontic tension exertion in rats may lead to masseter muscle atrophy,and prophylactic nitrate supple-mentation may resist hypoxia and atrophy of masseter muscle tissues by promoting angiogenesis through the anti-ap-optotic effects of the EGFR-AKT/ERK1/2 pathway.关键词
(牙合)位变化/咬肌萎缩/硝酸盐/低氧/血管生成/凋亡Key words
occlusal alterations/masseter muscle atrophy/nitrate/hypoxia/angiogenesis/apoptosis分类
医药卫生引用本文复制引用
刘围,刘小郁,吴婷婷..硝酸盐通过调控EGFR-AKT/ERK1/2信号通路预防咬肌萎缩[J].安徽医科大学学报,2025,60(8):1401-1409,9.基金项目
安徽省高校科研项目(编号:2022AH050734) （编号:2022AH050734）
安徽医科大学口腔医学院(附属口腔医院)学科建设"峰原"合作项目(编号:2022xkfyhz06、2022 xkfyhz03) Natural Science Research Project of Anhui Educational Committee(No.2022AH050734) （附属口腔医院）
Dis-cipline Construction "Fengyuan" Collaborative Projects of College & Hospital of Stomatology,Anhui Medical Uni-versity(Nos.2022xkfyhz06,2022xkfyhz03) （Nos.2022xkfyhz06,2022xkfyhz03）
