安徽医科大学学报2025，Vol.60Issue(8)：1410-1417,8.DOI:10.19405/j.cnki.issn1000-1492.2025.08.007
不同年龄段C57BL/6J雌性小鼠的行为及空间记忆变化
Changes in behavior and spatial memory of C57BL/6J mice of different ages
摘要
Abstract
Objective To explore the changes in behavior and spatial memory of C57BL/6J female mice of differ-ent ages(youth,middle-aged,and elderly).Methods C57BL/6J female mice were divided into female youth group(YG group),female middle-aged group(MG group)and female elderly group(OG group)according to age.The Morris water maze test measured spatial memory ability,and the open field and elevated cross maze test observed activity level and anxiety level.Western blot was used to determine the protein expressions of CREB,CaMK Ⅱ(pan)and CaMK Ⅱ(p)in the hippocampus of the brain tissues of female mice in each group.Results Compared with the YG group,the weight of the MG group and the OG group significantly increased(P＜0.01,P＜0.001).Compared with the OG group,the third quadrant escape latency and the number of crossings in the YG group and MG group were shortened,and the difference was not statistically significant.Compared with the OG group,there was a statistically significant difference in the exercise speed in the open field of the YG group(P＜0.01),there was no significant difference in the movement speed in the open field of the MG group,the number of entries into the central zone significantly increased in the MG group(P＜0.05),and there was no significant difference in the number of entries in the YG group(P＞0.05).Compared with the OG group,the YG group had a statistically significant difference in the elevated cross maze(P＜0.05),the MG group had no statistically signif-icant difference in the elevated cross maze,and the number of closed arm entries in the YG group and MG group significantly increased(P＜0.001,P＜0.01).Compared with the YG group,the relative expression level of CaMK Ⅱ(pan)in the OG group was statistically significant(P＜0.05),while the relative expression level of CaMK Ⅱ(pan)in the MG group was not statistically significant(P＞0.05).Conclusion With the increase of age,the weight of C57BL/6J female mice gradually increased,the activity level and desire to explore gradually de-creased,the spatial memory ability also declined,and the anxiety level and anxiety-like behavior increased.This study helps to reveal the effect of age on the activity level and cognitive function of females,and provides a refer-ence for studying cognitive and memory decline in older females.关键词
C57BL/6J小鼠/雌鼠/空间记忆/焦虑/CaMKⅡ(p)/行为学Key words
C57BL/6J mice/female rats/spatial memory/anxiety/CaMK Ⅱ(p)/ethology分类
医药卫生引用本文复制引用
杨振,钟健,张胜权,白红枚,胡韦康,李名聪,江小丽,张朝阳,王子涵,周文静,何清雅..不同年龄段C57BL/6J雌性小鼠的行为及空间记忆变化[J].安徽医科大学学报,2025,60(8):1410-1417,8.基金项目
安徽省自然科学基金项目(编号:2108085MH266) （编号:2108085MH266）
安徽省高校科研重点项目(自然)(编号:2022AH050651) （自然）
安徽医科大学校科研基金项目(编号:2022xkj107) （编号:2022xkj107）
蚌埠医学院科技项目自然科学重点项目(编号:2022byzd196) Natural Science Foundation of Anhui Province(No.2108085MH266) （编号:2022byzd196）
Natural Science Re-search Project of Anhui Educational Committee(Nature,Key Projict)(No.2022AH050651) （Nature,Key Projict）
Scientific Research Project of Anhui Medical University(No.2022xkj107) （No.2022xkj107）
Natural Science Project of Bengbu Medical University(No.2022byzd196) （No.2022byzd196）
