安徽医科大学学报2025，Vol.60Issue(8)：1423-1431,9.DOI:10.19405/j.cnki.issn1000-1492.2025.08.009
粪菌移植调节肠源性雌激素及其代谢物组成与非酒精性脂肪性肝病相关性研究
Fecal microbiota transplantation regulates the composition of intestinal-derived estrogens and their metabolites:a study on the correlation with non-alcoholic fatty liver disease
摘要
Abstract
Objective To investigate the impact of fecal microbiota transplantation(FMT)on the composition of 15 intestinal-derived estrogens and their metabolites(EMs)and its correlation with non-alcoholic fatty liver disease(NAFLD).Methods Thirty male C57BL/6J mice were divided into a normal control group(Control),a high-sugar high-fat diet combined with low-dose CCl4-induced NAFLD model group(Model),and a group of model mice treated with fecal microbiota from normal female mice(FMT).After 17 weeks of modeling,liver pathology in each group was observed using HE staining,biochemical methods were used to measure serum alanine aminotrans-ferase(ALT)and aspartate aminotransferase(AST)levels,as well as hepatic triglyceride(TG)and total choles-terol(TC)levels.and the content of 15 EMs in portal vein serum was detected using ultra-high-performance liquid chromatography-tandem mass spectrometry(UHPLC-MS).The correlation between disease phenotype and intesti-nal EMs was analyzed using Pearson's method.Results The NAFLD model was successfully established,and the FMT group showed improved liver structure and morphology,with significant decreases in liver function and hepatic lipids compared to the Model group.In NAFLD mice,the contents of E1,E2,and their 2-and 4-position metabo-lites in portal vein blood serum was reduced compared to normal mice,while the content of most 16-and 17-posi-tion metabolites(except 16α-OHE1)increased compared to normal mice.Correlation analysis showed that ALT was strongly positively correlated with E3 and 17-epiE3,and strongly negatively correlated with E1,E2,4-MeOE1,and 16α-OHE1.The TC was strongly positively correlated with 17-epiE3 and strongly negatively correla-ted with E1,4-MeOE1,and 16α-OHE1.Conclusion FMT restores the disrupted composition of intestinal EMs and improves NAFLD.关键词
非酒精性脂肪性肝病/肠道菌群/粪菌移植/雌激素/代谢组学Key words
non-alcoholic fatty liver disease/gut microbiota/fecal microbiota transplantation/estrogens/metabo-lomics分类
医药卫生引用本文复制引用
李婉丽,戚雪平,丛淑琪,张婉婷,张婷婷,汪生,方海明,文继月,王佳佳..粪菌移植调节肠源性雌激素及其代谢物组成与非酒精性脂肪性肝病相关性研究[J].安徽医科大学学报,2025,60(8):1423-1431,9.基金项目
国家自然科学基金面上项目(编号:82370591) （编号:82370591）
安徽省自然科学基金面上项目(编号:2308085MH242) （编号:2308085MH242）
安徽医科大学基础与临床合作研究提升计划项目(编号:2021xkjT023) National Natural Science Foundation of China(No.82370591) （编号:2021xkjT023）
Natural Science Foundation of Anhui Province(No.2308085MH242) （No.2308085MH242）
Basic and Clinical Collaborative Research Enhancement Project of Anhui Medical University(No.2021xkjT023) （No.2021xkjT023）
