动物医学进展2025，Vol.46Issue(11)：1-7,7.
插入序列共同区家族元件结构特征、参与捕获的耐药基因及启动子预测
Structure Characteristics of Insertion Sequence Common Region Family Elements,Involvement in Capturing Resistant Genes,and Promoter Prediction
摘要
Abstract
In order to predict and analyze scenarios where ISCR elements provide promoters for downstream resistance genes,sequence characteristics of identified ISCR family elements and their captured resistance genes were examined.Three classical IS91 family members(IS801,IS1294,IS91)and 17 publicly availa-ble ISCR sequences were included.Phylogenetic analysis of I SCR transposase amino acid sequences was performed using MEGA X,and variations within conserved motifs were aligned.Conserved nucleotide fea-tures of oriIS and terIS termini were compiled from literature,including characteristic base-pair patterns.Complete reference I SCR sequences were retrieved from the NCBI GenBank database,preliminarily annota-ted via the RAST platform,and resistance genes were verified using the Resfinder database.Intergenic re-gions between ISCR elements and downstream resistance genes were analyzed with CLC Genomics Work-bench 8.Promoter prediction was conducted using BPROM for 200 bp sequence upstream of each resistance gene.Cluster analysis demonstrated that IS28 and IS20 exhibit the closest evolutionary proximity to IS91 family members.While oriIS termini were highly conserved,terIS termini-excluding internal reverse-complementary sequences-lacked significant nucleotide conservation.ISCR elements facilitated the capture of at least 20 resistance genes,with eight cases suggesting that ISCR right termini provide functional pro-moters.These results indicate that ISCR elements not only mobilize resistance genes but may also drive their expression through promoter provision.This functional distinction from IS26,coupled with its status as the second most active insertion sequence family(after IS26)in resistance gene transfer,underscores its critical significance.关键词
ISCR元件/IS91家族元件/可移动元件Key words
ISCR element/IS91 family element/Mobile element引用本文复制引用
杜逸瑶,宿哲弟,王娟,袁敏,刘志国,邱小彤,徐帅,李振军..插入序列共同区家族元件结构特征、参与捕获的耐药基因及启动子预测[J].动物医学进展,2025,46(11):1-7,7.基金项目
国家自然科学基金项目(L2124006) （L2124006）
