动物医学进展2025，Vol.46Issue(11)：24-30,7.
PEDV和TGEV双重荧光重组酶介导等温扩增检测方法的建立及初步应用
Establishment and Preliminary Application of Duplex Fluorescence RAA Detection Method for PEDV and TGEV
摘要
Abstract
Primers and probes were designed based on the specific conserved sequences of the M gene of por-cine epidemic diarrhea virus(PEDV)and the S gene of porcine infectious gastroenteritis virus(TGEV),respectively,to establish a duplex fluorescence recombinase-mediated isothermal amplification(RAA)method for detecting PEDV and TGEV.This method can complete the detection at a constant temperature of 41 ℃ for 20 minutes,without cross reactivity with porcine coronavirus,porcine rotavirus,porcine circo-virus type 2,classical swine fever virus,and porcine pseudorabies virus.The minimum detection limits for PEDV and TGEV plasmid standards are 101 copies/μL and 103 copies/μL,respectively.The RT-qPCR de-tection method and the established fluorescent RAA detection method were used to detect 50 clinical sam-ples,and the positive coincidence rate of the two was 93.10％.The results indicated that the established duplex fluorescence RAA detection method for PEDV and TGEV has the potential to become an efficient diagnostic tool for veterinary clinical testing.关键词
荧光重组酶介导等温扩增检测/PEDV/TGEVKey words
Fluorescence recombinase aided amplification detection/PEDV/TGEV分类
农业科技引用本文复制引用
刘明妮,牟豪,吕林丹,李绍梅,高婕琪,杨柳..PEDV和TGEV双重荧光重组酶介导等温扩增检测方法的建立及初步应用[J].动物医学进展,2025,46(11):24-30,7.基金项目
国家生猪技术创新中心先导科技项目(NCTIP-XD/B11) （NCTIP-XD/B11）
重庆英才计划项目(cstc2022ycjh-bgzxm0183) （cstc2022ycjh-bgzxm0183）
