中国耳鼻咽喉头颈外科2025，Vol.32Issue(8)：496-501,6.DOI:10.16066/j.1672-7002.2025.08.005
小鼠慢性鼻窦炎模型组织免疫学特征分析及对糖皮质激素反应性研究
The analysis of the histological and immunological characteristics of the chronic rhinosinusitis model in mouse and the study of the response to glucocorticoids
刘苑君 1曹杰 2杨阳 3耿聪俐 4刘燕 4邢志敏4
作者信息
- 1. 首都医科大学附属北京安贞医院耳鼻咽喉头颈外科,北京 100029
- 2. 首都医科大学附属北京友谊医院耳鼻咽喉头颈外科,北京 100050
- 3. 北京积水潭医院耳鼻咽喉科,北京 100035
- 4. 北京大学人民医院耳鼻咽喉头颈外科,北京 100044
- 折叠
摘要
Abstract
OBJECTIVE To establish a reliable mouse eosinophilic chronic rhinosinusitis model,study the histological and immunological effects of glucocorticoids on type 2 inflammatory cells and factors in nasal mucosa,and provide an animal model basis for the biological treatment of CRS and related research of resistance to glucocorticoids in vivo.METHODS The CRS model group was treated with ovalbumin and Staphylococcal enterotoxins B.The dexamethasone intervention group received an additional weekly intraperitoneal injection of dexamethasone for 8 weeks.The expression of type-2 innate lymphoid cell(ILC2)in the nasal mucosa was detected with immunofluorescence and the expression of tryptase+mast cells,eosinophil cationic protein(ECP),IL-5,IL-25,and IL-33 in the nasal mucosa was detected with immunohistochemical.RESULTS The nasal mucosa eosinophils in the dexamethasone intervention group was lower than in the CRS model group(P<0.05).Thy-1+ST2+ILC2 cells in the dexamethasone intervention group were less than those of the CRS model group,and Thy-1+ST2+ILC2 cells were rare in the control group.Tryptase+mast cells were moderately positive in the CRS model group and weakly positive in the control group and in the dexamethasone intervention group.ECP and IL-5 were strongly positive in the CRS model group and weakly positive in the control group and the dexamethasone intervention group.IL-25 was moderately positive in the control group,strongly positive in the CRS model group,and weakly positive in the dexamethasone intervention group.IL-33 was weakly positive in the control group,while the CRS model group and the dexamethasone intervention group were strongly positive.CONCLUSION Intraperitoneal injection of dexamethasone reduce the number of eosinophils,Thy-1+ST2+ILC2 cells and tryptase+mast cells in the nasal mucosa of the mouse CRS model.It can also down-regulate the expression of ECP,IL-5,and IL-25 in tissues,but it does not have a significant effect on the expression of IL-33 in tissues.关键词
鼻窦炎/嗜酸细胞/白细胞介素类/糖皮质激素类/2型固有淋巴细胞/白细胞介素-33Key words
Sinusitis/Eosinophils/Interleukins/Glucocorticoids/type 2 innate lymphoid cells/interleukin-33引用本文复制引用
刘苑君,曹杰,杨阳,耿聪俐,刘燕,邢志敏..小鼠慢性鼻窦炎模型组织免疫学特征分析及对糖皮质激素反应性研究[J].中国耳鼻咽喉头颈外科,2025,32(8):496-501,6.
