中国耳鼻咽喉头颈外科2025，Vol.32Issue(8)：502-506,5.DOI:10.16066/j.1672-7002.2025.08.006
小鼠鼻腔细菌清扫方案比较
Comparison of bacterial clearance protocols in mouse nasal cavities
摘要
Abstract
OBJECTIVE To investigate the effectiveness of different bacterial cleaning methods and their effects on the general condition,nasal mucosal and systemic inflammation of mice.METHODS A total of 44 mice were randomly divided into six groups:4-antibiotic per os group(4ABX po)with 7 mice,5-antibiotic oral gavage group(5ABX og)with 8 mice,5-antibiotic intranasal group(5ABX in)with 8 mice,and each of their control groups with 7 mice.Body weight,water intake,and peripheral blood routine test of mice were measured.Bacterial culture of nasal lavage fluid(NLF)was performed;mRNA level of inflammatory mediators and histopathological analysis were conducted with mouse nasal mucosa.RESULTS Bacteria were cultured from all control groups,while Bacteria were cultured from all control groups,while one mouse in the 4ABX po group,three mice in the 5ABX og group,and two mice in the 5ABX in group showed no bacterial growth.The number of goblet cells in the nasal mucosa significantly increased in the 5ABX og group compared with its control group(P<0.05).The 5ABX in group exhibited significantly higher counts of peripheral blood lymphocytes and hemoglobin levels,as well as greater nasal mucosal thickness compared with its control group,with a notable decrease in goblet cells(P<0.05).No statistical differences were observed in body weight or the mRNA expression of nasal mucosal inflammatory mediators.CONCLUSION Different combinations of antibiotics and administration routes have varying effects on nasal bacteria,systemic and nasal mucosal inflammation in mice.Therefore,choosing appropriate protocols is crucial for the progression of subsequent research.关键词
小鼠/鼻腔/滴鼻/灌胃/抗生素Key words
Mice/Nasal cavity/intranasal/oral gavage/antibiotics引用本文复制引用
任益民,王成硕,刘向祎,李颖,梁爽,张桂,闫冰..小鼠鼻腔细菌清扫方案比较[J].中国耳鼻咽喉头颈外科,2025,32(8):502-506,5.基金项目
国家自然科学基金(82171108、82025010) （82171108、82025010）
北京市卫生系统高层次公共卫生人才建设项目(学科骨干-03-03) （学科骨干-03-03）
北京市卫生健康委员会市属医学科研院所公益发展改革试点项目(JYY2023-1) （JYY2023-1）
