渔业研究2025，Vol.47Issue(5)：583-590,8.DOI:10.14012/j.jfr.2025107
稀有鮈鲫GCRV共居感染模型的建立与评估
Establishment and evaluation of a co-habitation model for GCRV infection in Gobiocypris rarus
摘要
Abstract
[Background]Grass carp reovirus(GCRV)is the causative agent of hemorrhagic disease in grass carp(Ctenopharyngodon idella),posing a serious threat to freshwater aquaculture in China.Establishing an in-fection model that mimics natural transmission is essential for elucidating the epidemiology and pathogeny of this virus.[Objective]This study aims to develop a co-habitation model of GCRV infection in rare minnow(Gobiocypris rarus)to investigate its pathogenicity,viral load in tissues,and host immune responses.[Meth-ods]Rare minnows were infected with GCRV via intraperitoneal injection,and kept in the same tank with an equal number of healthy fish for up to 3 weeks.Disease progression was monitored,mortality was recorded,and survival rates were obtained.The expression levels of the viral gene(Vp5)encoding capsid protein and host an-tiviral gene(Mx1)in the gills,spleen,and hindgut were quantified using quantitative real-time polymerase chain reaction(qRT-PCR).[Results]In the injection group,mortality was observed on day 4 post-infection,and all injected fish died on day 8.In contrast,the co-habitation group exhibited a delayed and more prolonged disease progressing,with mortality recorded on day 12 and reaching 100%on day 20.It was shown that significantly higher Vp5 expression was detected in the gills compared to the spleen and hindgut under both infection modes.Additionally,the expression of Mx1 was significantly upregulated in all examined tissues.[Conclusion]A co-habitation infection model for GCRV was successfully established in rare minnow.The results indicated that gills serve as a key entry site for viral invasion.Although host antiviral defense is activated upon infection,it is insufficient to control viral replication.The co-habitation infection model provides a valuable tool for further in-vestigation into the transmission dynamics,pathogenesis,host immune response to GCRV,and the develop-ment of control strategies.关键词
草鱼呼肠孤病毒(GCRV)/草鱼/稀有鮈鲫/共居感染/水平传播Key words
grass carp reovirus(GCRV)/grass carp(Ctenopharyngodon idella)/rare minnow(Gobiocypris rarus)/co-habitation infection/horizontal transmission分类
农业科技引用本文复制引用
闫慧,高娃,陈康勇,冯建华,吴海霞,徐兢,徐镇,王俊亚,邹钧..稀有鮈鲫GCRV共居感染模型的建立与评估[J].渔业研究,2025,47(5):583-590,8.基金项目
国家自然科学基金(32030112) （32030112）
