渔业研究2025，Vol.47Issue(5)：598-608,11.DOI:10.14012/j.jfr.2025102
大黄鱼源贪食迈阿密虫(Miamiensis avidus)的分离鉴定、体外培养及药物敏感性研究
Isolation,identification,and in vitro characterization of Miamiensis avidus from large yellow croaker(Larimichthys crocea)
摘要
Abstract
[Background]The large yellow croaker(Larimichthys crocea)is a commercially valuable fish in China,widely farmed in marine aquaculture.However,rapid growth in intensive farming has led to poorer wa-ter quality and higher stocking densities,causing frequent disease outbreaks and economic losses.Notably,cili-ate protozoan infections,especially scuticociliatosis,pose a significant threat.[Objective]This study aimed to isolate and identify the ciliate causing scuticociliatosis in large yellow croaker and to understand its pathogenic and biological traits,providing a basis for disease prevention and control.[Methods]In April 2025,fish co-in-fected with trypanosomes and scuticociliates were collected from a net-cage farm in Ningde,China.The para-sites were isolated,cultured,and identified via morphological and molecular analysis.A stable culture system was developed to study the effects of temperature,pH,and salinity on ciliate growth.Drug sensitivity tests were also performed to evaluate common aquaculture anti-parasitics.[Results]Morphological and phylogenetic ana-lyses confirmed the ciliate as Miamiensis avidus.Temperature tests showed high sensitivity:all cells died at 0℃within 12 hours,and the population decreased at 30℃.Growth at 10℃was slower than at 20℃,with no significant difference at 25℃,indicating susceptibility to temperatures above 25℃.Salinity tests revealed all cells died at salinity 0 within 12 hours,while growth at salinity 10 was slower than at salinity 30,with no signi-ficant difference at salinity 20,indicating broad salinity tolerance.The parasite could grow at pH 5.5 and pH 9.0,though less than at pH 7.4,with no significant difference at pH 6.5 and pH 8.5,showing strong pH adaptab-ility.Drug sensitivity tests showed that carvacrol effectively eliminated M.avidus at a low dose(100 mg·kg-1),indicating its potential as a low-toxicity treatment.[Conclusion]The study confirms M.avidus as the cause of scuticociliatosis in sub-adult large yellow croaker,explores environmental factors affecting parasite growth,and identifies an effective low-dose treatment.These insights improve our understanding of M.avidus biology and support sustainable scuticociliatosis management in large yellow croaker aquaculture.关键词
大黄鱼/贪食迈阿密虫/种属鉴定/体外培养/环境因子/药敏实验Key words
large yellow croaker(Larimichthys crocea)/Miamiensis avidus/species identification/in vitro culture/environmental factors/drug sensitivity testing分类
农业科技引用本文复制引用
薛谦玺,郭香,袁家豪,韦海运,黄光亮,卓玉琛,陈新华,覃盼..大黄鱼源贪食迈阿密虫(Miamiensis avidus)的分离鉴定、体外培养及药物敏感性研究[J].渔业研究,2025,47(5):598-608,11.基金项目
国家重点研发计划项目(2022YFD2401001) （2022YFD2401001）
国家现代农业产业技术体系专项(CARS47-G19) （CARS47-G19）
福建省种业创新与产业化工程(2021FJSCZY01) （2021FJSCZY01）
