渔业研究2025，Vol.47Issue(5)：620-630,11.DOI:10.14012/j.jfr.2025090
贝类高致病性溶珊瑚弧菌关键毒力因子的鉴定与分析
Identification and analysis of key virulence factors in highly pathogenic Vibrio coralliilyticus infecting shellfish
摘要
Abstract
[Background]In recent years,frequent disease outbreaks during the shellfish nursery phase in China have caused substantial economic losses.Vibriosis is one of the primary pathogenic diseases involved,with Vibrio corallilyticus identified as a major pathogen in shellfish hatcheries in northern China,capable of causing acute mortality in bivalve larvae.[Objective]This study systematically investigates the physiological and biochemical characteristics of pathogenic strains,the proteomic profile and enzymatic activities of their ex-tracellular products(ECPs),aiming to identify key virulence factors that influence the pathogenicity of V.coral-lilyticus toward shellfish larvae.The findings are expected to provide a scientific basis for establishing an integ-rated prevention and control system for vibriosis during the shellfish nursery period in China.[Methods]Three highly pathogenic strains of V.corallilyticus(RSH02,RSH05,RSH08)isolated from shellfish hatcheries in Shandong and Jiangsu provinces were used in the study.The strains were cultured to the logarithmic growth phase,and growth curves were determined.ECPs from the three highly pathogenic strains and one low-patho-genicity strain were prepared simultaneously using the cellophane plate method.The molecular profiles of ECPs from different strains were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE)based on molecular sieving effects.Characteristic protein bands of the highly pathogenic strains were selected,and their extracellular protein compositions were identified using mass spectrometry.Finally,protease activity,hemolysin activity,and siderophore production were assessed using specific agar media(protease as-say medium,hemolysin identification medium,and chrome azurol s medium).[Results]All three highly patho-genic strains exhibited similar growth curves,with a logarithmic growth phase between 8 and 24 hours,and demonstrated strong proteolytic,hemolytic,and siderophore activities.The ECPs of RSH02,RSH05,and RSH08 were enriched with highly active aminopeptidase,peptidase M4,and neutral metalloproteinase,respect-ively.[Conclusion]Although the three highly pathogenic V.corallilyticus strains shared similar growth charac-teristics,their ECP compositions differed significantly,suggesting that different strains may exert high patho-genicity and tissue-lytic capability through the secretion of specific virulence factors,such as aminopeptidase,peptidase M4,and metalloproteinase.This finding provides new insights into the pathogenic mechanisms of V.corallilyticus.关键词
贝类育苗期/溶珊瑚弧菌/胞外产物/蛋白质谱Key words
shellfish hatchery stage/Vibrio coralliilyticus/extracellular products/protein profiles分类
农业科技引用本文复制引用
李培君,张翔,倪敬磊,黄博闻,王崇明,郭永军,白昌明..贝类高致病性溶珊瑚弧菌关键毒力因子的鉴定与分析[J].渔业研究,2025,47(5):620-630,11.基金项目
国家重点研发计划(2023YFD2400701) （2023YFD2400701）
中国水产科学研究院基本科研业务费专项(2023TD30) （2023TD30）
国家贝类产业技术体系(CARS-49) （CARS-49）
