渔业研究2025，Vol.47Issue(5)：631-639,9.DOI:10.14012/j.jfr.2025048
陵水才女虫自然感染程度对香港牡蛎凋亡及免疫相关基因表达的影响
The effects of Polydora lingshuiensis natural infection on the apoptotic and immune functions of Hong Kong oyster(Crassostrea hongkongensis)
摘要
Abstract
[Background]As an important economic mollusc along the coastal areas of South China,the Hong Kong oyster(Crassostrea hongkongensis)holds a crucial position in aquaculture and significantly contributes to the local fishery economy.Polydora spp.is a kind of common shell-boring parasites of C.hongkongensis.Severe infestation of Polydora spp.can cause the"black shell disease".The parasites even bore through the shell and invade the soft parts,triggering an immune response,which affecting the quality and economic value of C.hongkongensis.[Objective]To explore the effects of different Polydora spp.infestation degrees on the ap-optosis and immune-related genes of C.hongkongensis.[Methods]In this study,a total of 49 C.hongkongen-sis samples were collected from Hebao Island,Zhuhai City,and Guangdong Province.Firstly,the worms were identified by the morphological observations and molecular approach.Then,the image processing technology of Photoshop(PS)was used to count the percentage of the black shell areas of C.hongkongensis caused by the in-fection of Polydora spp.and classify the infection degrees.Combined with the real-time quantitative poly-merase chain reaction technology,comparison of the apoptosis and immune-related gene expression differences were carried out in the mantle and hepatopancreas tissues of C.hongkongensis with different Polydora spp.in-festation grades.[Results]The results showed that the worms were identified as Polydora lingshuiensis based on morphological and molecular charateristics.The percentage of the black shell areas within the right shell of C.hongkongensis caused by Polydora lingshuiensis infestation ranged from 4.07%to 53.59%.Among them,there were 0 sample with the infestation level of Grade Ⅰ,3 samples with Grade Ⅱ,25 samples with Grade Ⅲ,and 21 samples with Grade Ⅳ.Compared with the oyster's infestation at Grade Ⅱ,it was up-regulated in the apoptosis-related genes(Caspase-2,Caspase-3,Caspase-8,FasL,IAP,BAX)in the mantle and hepatopancreas of the oysters infested with Grade Ⅳ,and down-regulated in the immune-related genes(SABL,defension,lyso-zme,C3)were.The Spearman correlation analysis showed that the percentage of the black shell areas was posit-ively correlated with the expressions of Caspase-3 and Bax in the mantle and hepatopancreas,and negatively correlated with the expression of SABL,especially the positive correlation with the expression of Caspase-3 was particularly strong.[Conclusion]The percentage of the black shell areas can be used to assess the status of the C.hongkongensis,and it is related to the expression of apoptosis and immune genes.The study provides a theor-etical basis and exploration direction for the prevention and treatment of the"black shell disease"and the pro-tection of the development of C.hongkongensis aquaculture industry.关键词
陵水才女虫/香港牡蛎/外套膜/肝胰腺/凋亡/免疫反应Key words
Polydora lingshuiensis/Crassostrea hongkongensis/mantle/hepatopancreas/apoptosis/immune response分类
农业科技引用本文复制引用
付胜利,姜皓译,王玉香,卢洁,姚托,叶灵通..陵水才女虫自然感染程度对香港牡蛎凋亡及免疫相关基因表达的影响[J].渔业研究,2025,47(5):631-639,9.基金项目
国家现代农业产业技术体系(CARS-49) （CARS-49）
中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项(2023TS03) （2023TS03）
海南省自然科学基金(325QN511) （325QN511）
