佛山科学技术学院学报（自然科学版）2025，Vol.43Issue(5)：54-61,8.
β受体阻断剂卡维地洛对PRRSV-CHR6株体外增殖的影响
Effects of β-blocker carvedilol on the proliferation of PRRSV-CHR6 strain in vitro
摘要
Abstract
This study aimed to investigate the impact of the β-blocker Carvedilol(CAR)on the in vitro inhibition of Porcine reproductive and respiratory syndrome virus(PRRSV)proliferation.A series of experiments such as CCK8,Western blotting,RT-qPCR,and indirect immunofluorescence were conducted to explore the efficacy and mechanism of CAR on PRRSV.The findings revealed that CAR exhibited a CC50 of 30.33 μM and an IC50 of 0.15 μM,demonstrating significant inhibitory effects on PRRSV proliferation(P<0.05).Furthermore,at concentrations of 5 μM,10 μM,and 20 μM,CAR displayed effective anti-PRRSV activity(P<0.05).Both pre-treatment and post-treatment with CAR at a concentration of 5 μM demonstrated substantial anti-PRRSV effects(P<0.05).Additionally,treatment with CAR at a concentration of 5 μM significantly upregulated the mRNA transcription levels of IFN-β,ISG15,and ISG56,as well as the expression levels of phosphorylated IRF3(P<0.05),suggesting that CAR may activate the type I interferon signaling pathway to inhibit PRRSV proliferation.This study represents the first evidence for significant in vitro anti-PRRSV activity by the β-blocker CAR while also elucidating its mechanism against PRRSV through activation of the type I interferon signaling pathway.关键词
猪繁殖与呼吸综合征病毒/β受体阻断剂/卡维地洛/抗病毒作用Key words
Porcine reproductive and respiratory syndrome virus/beta-adrenergic blocker/carvedilol/antiviral effect分类
农业科技引用本文复制引用
李舜,于巧萍,邓静飞,孙倩,王一博,付强..β受体阻断剂卡维地洛对PRRSV-CHR6株体外增殖的影响[J].佛山科学技术学院学报（自然科学版）,2025,43(5):54-61,8.基金项目
广东省基础与应用基础研究基金资助项目(2022A1515140052) （2022A1515140052）
广东省重点建设学科科研能力提升资助项目(2022ZDJS036) （2022ZDJS036）
