医学分子生物学杂志2025，Vol.22Issue(5)：435-441,7.DOI:10.3870/j.issn.1672-8009.2025.05.004
过表达miR-193b-5p介导自噬逆转宫颈癌细胞顺铂化疗耐药的作用研究
Effect of Overexpression of miR-193b-5p on Autophagy to Reverse Cisplatin Chemotherapy Resistance in Cervical Cancer Cells
摘要
Abstract
Objective To investigate the effect and mechanism of overexpression of microRNA-193b-5p(miR-193b-5p)on cisplatin(DDP)-resistant cell line SiHa/DDP in human cervical cancer.Methods Human cervical cancer cell line(SiHa)and its cisplatin-resistant cell line(SiHa/DDP)were cultured.The survival rates of the two cell lines treated with DDP(5,10,15,20,25,30 μmol/L)were detected by MTT assay,and the half inhibitory concentration(IC50)value was determined.Cells were divided into 4 groups:SiHa group,SiHa/DDP group,mimics NC group(SiHa/DDP cells transfected with mimics NC),miR-193b-5p mimics group(SiHa/DDP cells transfected with miR-193b-5p mimics).The expression level of miR-193b-5p was detected by RT-qPCR.Cells were then treated with DDP,and the cell survival rate and apoptosis rate were detected by the MTT method and flow cytometry.The expression of microtubule-associated protein 1 light chain 3 B(LC3B)was detected by cell immunofluorescence staining,and the protein expres-sion levels of LC3 Ⅱ/LC3 Ⅰ,Beclin-1,and P62 were detected by Western blotting.Results The survival rate of SiHa/DDP cells was higher than that of the SiHa cells when treated with DDP at the same concentration(P＜0.05),and the IC50 value of cells was significantly increased(P＜0.05).The relative expression level of miR-193b-5p in the SiHa/DDP cells was significantly downregulated(P＜0.05).The relative expression level of miR-193b-5p in the miR-193b-5p mimics group was higher than that in the control group and mimics NC group(P＜0.05).Compared with those in the SiHa/DDP group and the mimics NC group,the survival rate of SiHa/DDP cells in the miR-193b-5p mimics group was significantly decreased,the apoptosis rate was significantly increased(P＜0.05),the fluorescence intensity of LC3 B in cells was significantly weakened,the LC3 Ⅱ/LC3 Ⅰ ratio and the relative expression level of Beclin-1 protein were significantly downregulated(P＜0.05),and the relative expression level of P62 protein was significantly upregulated(P＜0.05).Conclusion The expression level of miR-193b-5p was decreased in the cisplatin-resistant cell line SiHa/DDP of human cervical cancer.Its overexpression could reverse the resistance of SiHa/DDP cells to DDP,which may be related to its inhibition of cell autophagy.关键词
宫颈癌/微小RNA-193b-5p/顺铂/化疗耐药/自噬Key words
cervical cancer/microRNA-193b-5p/cisplatin/chemotherapy resistance/auto-phagy分类
医药卫生引用本文复制引用
热孜宛古丽·约麦尔,买买提·司马义,张晓玲,迪丽努尔·安外尔,周玲..过表达miR-193b-5p介导自噬逆转宫颈癌细胞顺铂化疗耐药的作用研究[J].医学分子生物学杂志,2025,22(5):435-441,7.基金项目
新疆维吾尔自治区卫生健康委"天山英才"医药卫生高层次人才项目(No.TSYC202401B147) This work was supported by a grant from the"Tianshan Talent"High-Level Medical and Health Professionals Project of the Health Commission of Xinjiang Uygur Autonomous Region(No.TSYC202401B147) （No.TSYC202401B147）
