医学分子生物学杂志2025，Vol.22Issue(5)：452-461,468,11.DOI:10.3870/j.issn.1672-8009.2025.05.006
鉴定EPDR1作为胃癌预后的生物标志物及其对胃癌细胞侵袭迁移的影响
Identification of EPDR1 as Prognostic Biomarker of Gastric Cancer and Its Effect on Invasion and Migration of Gastric Cancer Cells
梁晨希 1杨朝纲 1熊斌1
作者信息
- 1. 武汉大学中南医院胃肠外科 武汉市,430071||武汉大学中南医院肿瘤生物学行为湖北省重点实验室&湖北省肿瘤医学临床研究中心 武汉市,430071
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摘要
Abstract
Objective To analyze the expression level of recombinant ependymin-related pro-tein 1(EPDR1)in gastric cancer(GC)and its relationship with the prognosis of GC patients,and to further explore the effect of EPDR1 on the invasive and migrative abilities of GC cells and its potential mechanism.Methods The expression levels of EPDR1 in gastric cancer tissues and adja-cent non-cancerous tissues from the GSE62254 dataset of the GEO database and TCGA-GTEx were analyzed,and their associations with clinicopathological characteristics and prognosis of correspond-ing patients were investigated.Gene set enrichment analysis(GSEA)was used to explore the signa-ling pathways that EPDR1 might regulate in GC.The effects of EPDR1 on the migration and invasion of GC cells and its potential mechanism were investigated by RT-qPCR,Western blotting,immuno-fluorescence,and Transwell assay.Results Bioinformatics analysis showed that EPDR1 was signif-icantly overexpressed in both the primary and peritoneal metastases of GC,and its high expression was closely related to poor disease-free survival and overall survival of GC patients.GSEA analysis indicated that EPDR1 may participate in the occurrence and development of GC by activating the Wnt/β-catenin signaling pathway.In vitro cell experiments showed that overexpression of EPDR1 en-hanced the migrative and invasive abilities of GC cells,and activated the Wnt/β-catenin pathway to promote the up-regulation of CTNNB1 protein and endonuclear translocation.Conclusion EPDR1 is highly expressed in GC,which is closely related to the invasion,migration,and poor prognosis of GC,and can be used as a prognostic biomarker and potential therapeutic target for GC.关键词
胃癌/室管膜蛋白相关蛋白1/Wnt/β-catenin通路/预后/生物信息学分析Key words
gastric cancer/ependymin related protein 1/Wnt/β-catenin pathway/progno-sis/bioinformatics analysis分类
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梁晨希,杨朝纲,熊斌..鉴定EPDR1作为胃癌预后的生物标志物及其对胃癌细胞侵袭迁移的影响[J].医学分子生物学杂志,2025,22(5):452-461,468,11.
