医学分子生物学杂志2025，Vol.22Issue(5)：462-468,7.DOI:10.3870/j.issn.1672-8009.2025.05.007
lncRNA ANRIL靶向miR-324-5p对卵巢癌SKOV-3细胞侵袭及干细胞样特性的影响
Effect of lncRNA ANRIL on Invasion and Stem Cell-like Characteris-tics of Ovarian Cancer Cells Skov-3 by Targeting miR-324-5p
摘要
Abstract
Objective To elucidate the molecular mechanism of lncRNA ANRIL in regulating the invasion and stem cell-like characteristics of ovarian cancer,and to analyze its dual role in me-diating chemotherapy resistance and tumor recurrence.Methods ANRIL stable knockdown SKOV-3 cell model was established(Control group,shRNA-NC group,ANRIL-shRNA1 group).The abili-ty of cell invasion and metastasis was evaluated by Transwell assay,wound-healing assay,and the expression of VEGF and Fibronectin The characteristics of stem cells were analyzed by tumor sphere formation assay combined with stem-cell marker CD44/SOX2/OCT4 detection The targeting interac-tion between ANRIL-shRNA1 and miR-324-5p was verified by dual luciferase reporter assay.A func-tional recovery experiment(ANRIL-shRNA1+miR-324-5p inhibitor co-transfection group)was designed to clarify the mediating effect of miR-324-5p on the regulation of VEGF and CD44 by AN-RIL-shRNA1.Results ANRIL knockdown decreased the invasion and migration ability of SKOV-3 cells,and the expression levels of VEGF and Fibronectin were significantly down-regulated.At the same time,the number of spheres was decreased,and the expression levels of CD44,SOX2,and OCT4 were decreased significantly compared with those in the control group.Dual luciferase assay confirmed that ANRIL directly binded to miR-324-5p.The inhibition of miR-324-5p could reverse the inhibitory effect of ANRIL knockdown on VEGF and CD44.Conclusion LncRNA ANRIL can pro-mote cell invasion and help maintain the characteristics of tumor stem cells by targeting miR-324-5p.关键词
卵巢癌/lncRNA ANRIL/miR-324-5p/肿瘤干细胞Key words
ovarian cancer/lncRNA ANRIL/miR-324-5p/cancer stem cells分类
医药卫生引用本文复制引用
朱蓓蓓,蒋艳丽,郭敏,杜卓..lncRNA ANRIL靶向miR-324-5p对卵巢癌SKOV-3细胞侵袭及干细胞样特性的影响[J].医学分子生物学杂志,2025,22(5):462-468,7.基金项目
陕西省自然科学基金(No.2021JD1003) This work was supported by a grant from the Shaanxi Province Natural Science Foundation(No.2021JD1003) （No.2021JD1003）
