医学分子生物学杂志2025，Vol.22Issue(5)：469-475,7.DOI:10.3870/j.issn.1672-8009.2025.05.008
基于PI3K/AKT/Bax通路探讨丁酸钠改善脓毒症急性肾损伤的实验研究
Sodium Butyrate Improves Acute Kidney Injury in Sepsis Through PI3K/AKT/Bax Pathway
摘要
Abstract
Objective To explore the effect and mechanism of sodium butyrate(NaB)on a-cute kidney injury(AKI)in mice with sepsis.Methods A sepsis AKI model was prepared in 30 mice and randomly divided into 3 groups:model group,NaB low-dose(NaB-L)group,NaB high-dose(NaB-H)group,with 10 mice in each group.Another 10 mice were taken as the Sham group.HE staining was used to observe the morphological changes in the kidneys of mice.Automatic biochemical analyzer was used to detect the levels of serum creatinine(Scr)and blood urea nitro-gen(BUN).The chemiluminescence method was used to determine the levels of serum kidney inju-ry molecule-1(KIM-1).The ELISA method was used to determine the levels of inflammatory factors such as interleukin(IL)-1 β,IL-6,and tumor necrosis factor-α(TNF-α)in renal tissues of mice.TUNEL staining was used to observe the apoptosis in renal tissue cells of mice.Western blot-ting and immunohistochemical staining were used to detect the expression of proteins related to the phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)/B cell lymphoma-2 protein(Bcl)associated X protein(Bax)pathway in renal tissues of mice in each group.Results Com-pared with the Sham group,the model group showed swollen,detached,and necrotic renal tubular epithelial cells,and interstitial hemorrhage accompanied by obvious inflammatory cell infiltration,and glomerular expansion or atrophy.The renal injury score,the levels of Scr,BUN and KIM-1 were increased in the model group(P＜0.05),the levels of IL-1 β,IL-6 and TNF-α in renal tis-sues were increased(P＜0.05),the proportion of TUNEL-positive cells was increased(P＜0.05),the expression of p-PI3K,p-AKT and Bcl-2 were decreased(P＜0.05),and the expres-sion of Bax was increased(P＜0.05).Compared with those in the model group,the lesions of renal tubular,interstitial and glomerular in mice in the NaB-L and NaB-H groups were alleviated,the re-nal injury score,the levels of Scr,BUN and KIM-1 were reduced(P＜0.05),the levels of IL-1 β,IL-6 and TNF-α in renal tissues were reduced(P＜0.05),the proportion of TUNEL-positive cells was reduced(P＜0.05),and the expression of p-PI3K,p-AKT and Bcl-2 were increased(P＜0.05),and the expression of Bax was decreased(P＜0.05).Conclusion NaB can improve renal injury in septic mice,and its mechanism may be related to the activation of PI3K/Akt signa-ling pathway and the inhibition of Bax expression.关键词
脓毒症/急性肾损伤/丁酸钠Key words
sepsis/acute kidney injury/sodium butyrate分类
医药卫生引用本文复制引用
王博青,李岩,杨健,罗彬..基于PI3K/AKT/Bax通路探讨丁酸钠改善脓毒症急性肾损伤的实验研究[J].医学分子生物学杂志,2025,22(5):469-475,7.基金项目
新疆医科大学第五附属医院青年科研启航项目(No.XYDWFY-ZR-202306),新疆维吾尔自治区卫生健康青年医学科技人才专项科研项目(No.WJWY-202434) This work was supported by grants from the Youth Research Voyage Project of the Fifth Affiliated Hospital of Xinjiang Medical University(No.XYDWFY-ZR-202306),Special Research Project for Health and Health Young Medical Science and Technology Talents in Xinjiang Uygur Autonomous Region(No.WJWY-202434) （No.XYDWFY-ZR-202306）
