南方农业学报2025，Vol.56Issue(7)：2062-2079,18.DOI:10.3969/j.issn.2095-1191.2025.07.003
叉角厉蝽消化相关蛋白酶基因家族鉴定及比较分析
Identification and comparative analysis of digestion-related gene families in Eocanthecona furcellata
摘要
Abstract
[Objective]This study aimed to elucidate the evolutionary characteristics of key genes in digestion-related proteases in Eocanthecona furcellata and their adaptive mechanisms to predatory feeding habits,so as to provide a theo-retical foundation for artificial mass rearing and biocontrol applications of Eocanthecona furcellata.[Method]Two preda-tory stink bugs(Arma custos and Eocanthecona furcellata)and two phytophagous stink bugs(Nezara viridula and Riptor-tus pedestris)belonged to Hemiptera:Pentatomomorpha were used to identified gene families of three digestion-related proteases(aspartic proteases,serine proteases,and cysteine proteases)across the four species using genome data.Key genes were screened through phylogenetic tree,collinearity analysis,and protein sequence conservation analysis.The ex-pression of key digestion-related protease genes during different developmental stages were detected via quantitative re-verse transcription PCR(qRT-PCR).Weighted gene co-expression network analysis(WGCNA)was employed to explore the gene expression patterns of Eocanthecona furcellata across developmental stages and delineate the modular regulatory networks of digestion-related protease genes.[Result]Phylogenetic analysis of the digestion-related gene families of the four Hemiptera insects revealed that digestion-related protease genes were classified into three gene families,which were serine protease,cysteine protease,and aspartic protease families.Eocanthecona furcellata exhibited significant ortholo-gous relationships with Arma custos in digestive protease genes(excluding self-clustering nodes),while Nezara viridula showed closer orthologous relationships with Riptortus pedestris.Based on previous literature and observations during the rearing of Eocanthecona furcellata,combined with heatmaps of expression patterns in development stages of Eocan-thecona furcellata,six key digestion-related protease genes were selected and designated as EfAP1,EfCP1,EfCP2,EfSP1,EfSP2,EfSP3.qRT-PCR results demonstrated that:the six genes were not expressed at egg stage;at the 1st instar nymph stage,the relative expressions of the selected genes were significant higher than those at 2nd to 5th instar nymph stages and adult stage(P<0.05)except for EfCP2(P>0.05).Such results were consistent with the change patterns of FPKM of transcriptomic data.WGCNA analysis identified 35 co-expression modules,with aspartic pro-tease genes were predominantly clustered in the MEyellow module,cysteine protease genes in the MEturquoise module,and serine prote-ase genes in the MEblue module,indicating that different protease genes possess independent regulatory networks.[Con-clusion]This study preliminarily reveals the evolutionary characteristics of digestion-related protease genes,identifies,and screens three digestion-related protease gene families and six key digestive protease genes for Eocanthecona furcel-lata.The modular co-expression patterns of these genes suggest that the six genes are close related to adaptation to preda-tory feeding habits of Eocanthecona furcellata.关键词
叉角厉蝽/捕食性昆虫/比较分析/食性适应机制Key words
Eocanthecona furcellata/predatory insects/comparative analysis/feeding adaptation mechanisms分类
农业科技引用本文复制引用
高有寿,赖昭渭,廖仁涛,丁以纾,张嘉欣,蔡明洋,刘正杰,毛自朝..叉角厉蝽消化相关蛋白酶基因家族鉴定及比较分析[J].南方农业学报,2025,56(7):2062-2079,18.基金项目
国家自然科学基金项目(32260482) （32260482）
中国烟草总公司云南省公司科技项目(2023530000241012) National Natural Science Foundation of China(32260482) （2023530000241012）
Science and Technology Program of Yunnan Provincial Company of China Tobacco Corporation(2023530000241012) （2023530000241012）
