南方农业学报2025，Vol.56Issue(7)：2102-2112,11.DOI:10.3969/j.issn.2095-1191.2025.07.006
不同食物对大草蛉生长发育和繁殖的影响及转录组比较分析
Effects of different foods on growth,development and reproduction of Chrysopa pallens and comparative transcriptome analysis
摘要
Abstract
[Objective]This study aimed to analyze the effects of different foods on the development,reproduction and transcriptomic differences of Chrysopa pallens,to screen indoor alternatives to feed Chrysopa pallens larvae,so as to provide a theoretical basis for large-scale Chrysopa pallens rearing.[Method]Four food types-Aphis craccivora nymphs,Sitobion avenae nymphs,2nd instar larvae of Spodoptera litura,2nd instar larvae of Spodoptera frugiperda were used to feed the 1st,2nd,3rd instar larvae of Chrysopa pallens under indoor artificial rearing conditions to investigate the effects of different foods and rearing stages on the development and reproduction of Chrysopa pallens.The transcriptome sequen-cing was performed on the 3rd instar larvae fed on Aphis craccivora and Spodoptera frugiperda based on effects of different foods on biological characteristics of Chrysopa pallens.[Result]During the three rearing stages,Chrysopa pallens larvae fed on Aphis craccivora and Sitobion avenae could develop and pupate normally.Low survival rates in pupal stage were observed among the Chrysopa pallens larvae fed on Spodoptera litura and Spodoptera frugiperda since their 1st and 2nd instar stages;however,for Chrysopa pallens fed on these two food types since 3rd instar stage,the feeding effects on their development was less pronounced as most Chrysopa pallens larvae were able to pupate.For the 3rd instar larvae of Chrysopa pallens,no significant effects of feeding on the four food types was found in their pre-oviposition period or ovi-position duration of females(P>0.05),but those fed on Aphis craccivora and Sitobion avenae enjoyed a significantly higher fecundity per female and egg hatching rates than those fed on Spodoptera litura and Spodoptera frugiperda(P<0.05).Transcriptomic analysis revealed 680 differentially expressed genes(DEGs)between Chrysopa pallens larvae fed on Spodoptera frugiperda and those fed on Aphis craccivora,among which 333 were up-regulated and 347 were down-regulated.GO functional annotation and enrichment analysis indicated that these DEGs were primarily enriched in terms related to integral component and intrinsic component of membrane.KEGG signal pathway enrichment analysis showed that the DEGs were mainly enriched in pathways such as metabolism of xenobiotics by cytochrome P450,ascor-bic acid and aldarate metabolism.[Conclusion]Chrysopa pallens larvae of all instar stages predate Aphis craccivora nymphs,Sitobion avenae nymphs,the 2nd instar larvae of Spodoptera litura,and the 2nd instar larvae of Spodoptera frugi-perda.As Chrysopa pallens larvae grow with stronger adaptability to different foods,feeding on Spodoptera litura and Spodoptera frugiperda since the 3rd instar stage brings minor effects on their growth,development and reproduction.DEGs identified between Chrysopa pallens fed on Spodoptera frugiperda and those fed on Aphis craccivora are associated with material transport,cellular immunity,and energy metabolism.关键词
大草蛉/自然猎物/生长发育/转录组Key words
Chrysopa pallens/natural prey/development/transcriptome分类
农业科技引用本文复制引用
方洁,肖艳松,史子涵,左昌昊,智磊,周观星,包强,谭琳..不同食物对大草蛉生长发育和繁殖的影响及转录组比较分析[J].南方农业学报,2025,56(7):2102-2112,11.基金项目
湖南省重点研发计划项目(2023NK2013) （2023NK2013）
湖南省烟草公司郴州市公司科技项目(CZYC2023JS06) Hunan Province Key Research and Development Project(2023NK2013) （CZYC2023JS06）
Science and Techno-logy Project Chenzhou Branch,Hunan Province Tobacco Company(CZYC2023JS06) （CZYC2023JS06）
