海南大学学报（自然科学版）2025，Vol.43Issue(4)：405-415,11.DOI:10.15886/j.cnki.hndk.2024121102
蒽酮法测定海菖蒲叶片可溶性糖含量的条件优化
Condition optimization of the anthrone-sulfuric acid method for soluble sugar content determining in Enhalus acoroides leaves
摘要
Abstract
In the report,adjusting factors such as anthrone solution dosage,reaction temperature,and reaction time was performed.The anthrone sulfuric acid method were systematically optimized to detect the soluble sugar content in seagrass leaves.Based on these parameters,the effects of four drying methods(60 ℃ drying,air drying,silica gel drying,and freeze drying)and two extraction methods(water bath extraction and ultrasonic extraction)on soluble sugar extraction were compared and analyzed.The results indicated that the optimal sensitivity and accuracy were achieved when 0.20 mL of sample extract was combined with 1.00 mL of anthrone-sulfuric acid solution,the reaction temperature was 95℃,the reaction time was 6.5 minutes,and the detection wave length was 624 nm.The extraction efficiency was obtained when drying at 60℃ was followed by water bath extraction.The method is simple to execute,stable,and requires a minimal sample size,which makes it ideal for the rapid analysis of large-scale seagrass samples and offers the reliable technical support for the ecological research on seagrass.关键词
海草/海菖蒲/可溶性糖/蒽酮-硫酸法/方法优化Key words
seagrass/Enhalus acoroides/soluble sugar/anthrone-sulphuric acid method/method optimization分类
海洋科学引用本文复制引用
邹留源,王杰,覃乐政,王凤阳..蒽酮法测定海菖蒲叶片可溶性糖含量的条件优化[J].海南大学学报（自然科学版）,2025,43(4):405-415,11.基金项目
国家自然科学基金项目(42206152) （42206152）
