桑葚花青素的提取及纯化工艺OA

Based on the single-factor experiment results of extracting anthocyanins from mulberry flowers with ultrasonic-assisted organic solvents,the response surface methodology was used to optimize the extraction process parameters.Mean-while,the adsorption and desorption parameters for purifying the crude anthocyanin extract were explored in combination with macroporous resins.The results showed that the optimal extraction conditions were ethanol concentration of 80%,extraction time of 30 min,liquid to solid ratio of 1∶40,extraction temperature of 50℃,and the extraction amount of mulberry anthocya-nins was 11.85 mg/g.Among the seven macroporous resins,D280 was the best.The static adsorption and desorption time were both 6 hours.The sample loading volume was 3 BV,and the sample loading concentration was 281.646 mg/L.The desorption solution was 70%ethanol,with a volume of 4 BV and pH=2.After purification,the color value of mulberry anthocyanidins in-creased by 8.5 times,and the purity increased from 16.22%to 83.78%.Therefore,higher purity of mulberry anthocyanidins could be obtained by ultrasonic-assisted organic solvent extraction and macroporous resin purification.