海南师范大学学报（自然科学版）2025，Vol.38Issue(3)：308-315,8.DOI:10.12051/j.issn.1674-4942.2025.03.008
桑葚花青素的提取及纯化工艺
Extraction and Purification Process of Anthocyanidin from Mori Fructus
摘要
Abstract
Based on the single-factor experiment results of extracting anthocyanins from mulberry flowers with ultrasonic-assisted organic solvents,the response surface methodology was used to optimize the extraction process parameters.Mean-while,the adsorption and desorption parameters for purifying the crude anthocyanin extract were explored in combination with macroporous resins.The results showed that the optimal extraction conditions were ethanol concentration of 80%,extraction time of 30 min,liquid to solid ratio of 1∶40,extraction temperature of 50℃,and the extraction amount of mulberry anthocya-nins was 11.85 mg/g.Among the seven macroporous resins,D280 was the best.The static adsorption and desorption time were both 6 hours.The sample loading volume was 3 BV,and the sample loading concentration was 281.646 mg/L.The desorption solution was 70%ethanol,with a volume of 4 BV and pH=2.After purification,the color value of mulberry anthocyanidins in-creased by 8.5 times,and the purity increased from 16.22%to 83.78%.Therefore,higher purity of mulberry anthocyanidins could be obtained by ultrasonic-assisted organic solvent extraction and macroporous resin purification.关键词
桑椹花青素/提纯/大孔树脂/响应面法Key words
mulberry anthocyanin/purification/macroporous resin/response surface methodology分类
农业科技引用本文复制引用
潘禹澄,沈玲,王雅静,邵玟菲,贾光锋,孙飞龙..桑葚花青素的提取及纯化工艺[J].海南师范大学学报（自然科学版）,2025,38(3):308-315,8.基金项目
陕西省大学生创新训练计划项目(202410709067) （202410709067）
陕西省重点研发计划项目(2023-YBNY-167) （2023-YBNY-167）
