湖南中医药大学学报2025，Vol.45Issue(9)：1663-1669,7.DOI:10.3969/j.issn.1674-070X.2025.09.010
推拿按法调节巨噬细胞极化缓解慢性激痛点模型大鼠疼痛的效应研究
Effects of tuina pressing manipulation in regulating macrophage polarization to alleviate pain in chronic myofascial trigger point model rats
摘要
Abstract
Objective To investigate the effects of tuina pressing manipulation in regulating M1/M2 macrophage polarization for the treatment of chronic myofascial trigger point(MTrP)model rats.Methods Thirty SPF-grade male SD rats were randomized into three groups:a blank group,a model group,and a pressing manipulation group,with 10 rats in each group.Chronic MTrP models were induced in the left medial thigh muscles of rats in the model and pressing manipulation groups,using blunt impact combined with eccentric exercise.After modeling,rats in the pressing manipulation group received local stimulation of MTrPs with tuina pressing manipulation.The mechanical pressure pain threshold(PPT)at the MTrPs was measured using a pressure analgesia-meter;soft tissue tension was checked with a soft tissue tension meter.Histopathological morphological changes in MTrP muscle tissues were observed via HE staining;inflammatory cytokines,including pro-inflammatory cytokines interleukin(IL)-1β and tumor necrosis factor-α(TNF-α),as well as anti-inflammatory cytokines IL-4 and IL-10,were examined using ELISA.Protein expressions of CD86 and CD206 in MTrP muscle tissues were measured by Western blot and immunofluorescence assays.Results Compared with the blank group,the model group exhibited a decrease in PPT(P<0.05)and an increase in soft tissue tension(P<0.05).The levels of IL-1β and TNF-α,as well as CD86 protein expression,were elevated(P<0.05),while the IL-4 and IL-10 levels,along with CD206 protein expression,were reduced(P<0.05).In contrast,the pressing manipulation group showed an increase in PPT(P<0.05)and a decrease in soft tissue tension(P<0.05)compared with the model group.The levels of IL-1β and TNF-α and CD86 protein expression were reduced(P<0.05),whereas the IL-4 and IL-10 levels and CD206 protein expression were elevated(P<0.05).Conclusion Tuina pressing manipulation can suppress local inflammation,alleviate pain,and repair damaged muscle tissues in chronic MTrP model rats by promoting M2 macrophage polarization and inhibiting M1 macrophage polarization.关键词
炎性疼痛/激痛点/推拿按法/巨噬细胞极化/压痛阈值/炎症因子Key words
inflammatory pain/myofascial trigger points/tuina pressing manipulation/macrophage polarization/pressure pain threshold/inflammatory cytokines分类
医药卫生引用本文复制引用
闪逸仙,唐丽亚,潘杰灵,张玉乔,齐映侠,李江山,陈敏,李武..推拿按法调节巨噬细胞极化缓解慢性激痛点模型大鼠疼痛的效应研究[J].湖南中医药大学学报,2025,45(9):1663-1669,7.基金项目
国家自然科学基金项目(82174526,82274676,82374613,82474669) （82174526,82274676,82374613,82474669）
湖南省科技创新计划资助项目(2022RC1221),湖南中医药大学研究生创新课题(CX20240733). （2022RC1221）
