湖南农业大学学报（自然科学版）2025，Vol.51Issue(4)：15-24,10.DOI:10.13331/j.cnki.jhau.2025.04.003
辣椒SEP亚家族基因鉴定及CaSEP3表达分析
Identification of SEP subfamily and expression analysis of CaSEP3 in pepper
摘要
Abstract
Six coding genes of SEPALLATA(SEP)subfamily members were identified through genome-wide analysis of the ST-8 Zhangshugang pepper.The physicochemical properties,chromosome localization,encoded protein conserved motifs and conserved structures of the subfamily members were analyzed.The phylogenetic tree was constructed with the SEP genes of pepper,Arabidopsis,tomato,rice and maize.Furthermore,tissue expression level of CaSEP3 and subcellular localization of encoded protein were explored.The results showed that SEP genes of pepper were distributed on five chromosomes,with all their encoded protein contained MADS domain(MADS_MEF2_like)and K-box domain.SEP genes could be divided into two branches:SEP3 and SEP1/2/4,which was similar to the evolutionary development of tomato and Arabidopsis.CaSEP3 was a homologous protein of AtSEP3,which was localized in the nucleus.CaSEP3 was highly expressed in flower buds,placenta and pericarp,which was speculated to be involved in development of flower and fleshy fruit in pepper.After common hormone and abiotic stress treatments(0～24 h),the expression of CaSEP3 was up-regulated by ABA,H2O2 and NaCl,while the expression of CaSEP3 in leaves and roots did not change significantly under other treatments.关键词
辣椒/花发育/SEPALLATA亚家族/生物信息学/表达分析Key words
pepper/floral development/SEPALLATA subfamily/bioinformatics/expression analysis分类
农业科技引用本文复制引用
梁心仪,汤冰倩,张新昊,邹学校,刘峰..辣椒SEP亚家族基因鉴定及CaSEP3表达分析[J].湖南农业大学学报（自然科学版）,2025,51(4):15-24,10.基金项目
特色蔬菜产业技术体系项目(CARS-24-A-15) （CARS-24-A-15）
