湖南农业大学学报（自然科学版）2025，Vol.51Issue(4)：38-47,10.DOI:10.13331/j.cnki.jhau.2025.04.005
紫花苜蓿MsWRKY47基因的克隆及耐盐功能分析
Cloning and functional analysis of MsWRKY47 gene in alfalfa under salt stress
摘要
Abstract
In order to clarify the role of MsWRKY47 gene of alfalfa(Medicago sativa)in regulating salt tolerance in plants,the MsWRKY47 gene was cloned using the cDNA of Medicago sativa'WL363 HQ'as a template,and its protein sequence characteristics and the salt tolerance of MsWRKY47 transgenic Arabidopsis thaliana were analyzed.The results showed that the full length of MsWRKY47 coding region was 1 287 bp,which encoded 428 amino acids and contained a WRKYGQK domain,which belonged to WRKY family and was closely related to homologous protein MtWRKY47 in Medicago truncatula.The expression of MsWRKY47 was the highest in alfalfa roots and could be induced by salt stress.MsWRKY47 was localized in nucleus and had no transcriptional activation activity.The yellow leaf rate of transgenic plants was higher than that of wild type after salt stress,and the oxidative damage was more serious,indicating that the overexpression of MsWRKY47 gene reduced the salt tolerance of Arabidopsis.MsWRKY protein may play a negative regulatory role in the response of plants to salt stress.关键词
紫花苜蓿/WRKY转录因子/盐胁迫/功能分析Key words
alfalfa/WRKY transcription factor/salt stress/functional analysis分类
农业科技引用本文复制引用
严帅凤,赵梦冉,徐倩,卢蕊,胡龙兴..紫花苜蓿MsWRKY47基因的克隆及耐盐功能分析[J].湖南农业大学学报（自然科学版）,2025,51(4):38-47,10.基金项目
国家自然科学基金项目(32201456,32071877) （32201456,32071877）
