基础医学与临床2025，Vol.45Issue(10)：1291-1297,7.DOI:10.16352/j.issn.1001-6325.2025.10.1291
松果菊苷减轻乙型肝炎模型大鼠肝损伤
Echinacoside attenuates liver injury in a rat model of hepatitis B
摘要
Abstract
Objective To investigate the effect of Echinacoside(Ech)on liver injury in hepatitis B rats.Methods SD rats were divided into control group,Hepatitis B(HBV,5 mL/kg HBV virus was injected into the tail vein twice a week for 3 consecutive weeks),low(Ech-L)and high(Ech-H)doses of Ech were injected into the stom-ach for HBV(8.33 and 33.32 mg/kg,respectively),HBV group was treated with positive drugs(lamivudine,10 mg/kg)and Ech-H+BKM120(40 mg/kg BKM120),with 12 animals in each group.The drug was administered once a day for 8 weeks.The activity of ALT and AST in serum of were detected.Serum level of gam-ma-interferon(IFN-γ)and interleukin(IL-4)was detected by ELISA;Flow cytometry was applied to detect Th1/Th2 in peripheral blood;Chromatin immunoprecipitation was applied to detect HBV DNA expression in liver tissue;HE staining was used to detect the pathological changes of liver tissue and score the damage;Western blot was ap-plied to detect phosphorylated phosphatidylinositol 3 kinase(p-PI3K),phosphorylated protein kinase B(p-AKT)proteins in liver tissue.Results Compared with the control group,hepatocytes in the hepatitis B group were swollen,nuclear staining was excessive,hepatic lobular structure was disorganized,pathological injury score,serum AST,ALT activity,IL-4 level,peripheral blood Th2,HBV DNA expression in liver tissue were all increased.Serum IFN-γ level,Th1,Th1/Th2 in peripheral blood,p-PI3K and p-AKT protein in liver tissue were all decreased(P＜0.05);Treatment with Ech-L,Ech-H and lamivudine reduced the swelling of HBV rat hepatocytes,cleared the structure of some liver cords,reduced pathological injury score,the activity of AST,ALT and IL-4 levels in serum,Th2 in peripheral blood and HBV DNA in liver tissue;The level of serum IFN-γ,Th1,Th1/Th2 in peripheral blood,and protein expression p-PI3K and p-AKT protein in liver tissue were increased(P＜0.05).BKM120 attenuated the ameliorative effect of high-dose Ech on hepatitis B-induced liver injury in rats.Conclusions Ech ameliorates liver in-jury in rats with hepatitis B,and the mechanism may be related to the activation of the PI3K/AKT pathway.关键词
松果菊苷/磷脂酰肌醇3激酶/蛋白激酶B通路/乙型肝炎/肝损伤/免疫Key words
echinacoside/phosphatidylinositol-3 kinase/protein kinase B pathway/hepatitis B/liver injury/immune分类
医药卫生引用本文复制引用
王维,穆宝龙,张文双,吴清雷..松果菊苷减轻乙型肝炎模型大鼠肝损伤[J].基础医学与临床,2025,45(10):1291-1297,7.基金项目
河北省2024年度医学科学研究课题计划(20241127) （20241127）
