极地研究2025，Vol.37Issue(3)：541-550,10.DOI:10.13679/j.jdyj.20240053
高通量微液滴技术在南大洋沉积物微生物分离培养中的应用研究
Application and culture efficiency of the high-throughput microdroplet technique in the isolation and cultivation of microorganisms from Southern Ocean sediments
摘要
Abstract
To evaluate the feasibility of microdroplet technology for the isolation and cultivation of polar micro-organisms,and to explore its potential for enhancing microbial cultivation efficiency,this study utilized high-throughput microdroplet encapsulation technology to conduct research on the isolation and cultivation of microorganisms from sediment samples collected in the Southern Ocean.The results show that:(1)Compared with the traditional spread plate method,differences were observed in the bacterial groups iso-lated and cultivated using the high-throughput microdroplet method.Although both methods primarily iso-lated Halomonas,the resultant proportions differed.(2)Both methods successfully isolated other strain groups that the other method failed to cultivate.This indicates that the combined use of these two techniques can effectively increase microbial species diversity.(3)Under the conditions of this study,the total number of strains isolated and cultured by the high-throughput microdroplet method was less than that isolated by the traditional spread plate method,but more non-redundant amplicon sequence variants were detected with the high-throughput method than with the traditional spread plate method per unit time,which reflects the potential of the high-throughput method for improving the efficiency of microbial separation.This study provides a scientific basis for exploring the application of high-throughput microdroplet encapsulation technology in the isolation and cultivation of polar microorganisms and offers insight and a reference for further optimization of such methods in the isolation and cultivation of polar microorganisms in future stud-ies.关键词
高通量微液滴技术/菌株分离培养/群落结构/沉积物微生物/南大洋Key words
high-throughput microdroplet technique/strain isolation and cultivation/community structure/sediment microorganisms/Southern Ocean引用本文复制引用
刘忆思,张瑾,毛梦婷,刘仁宗,段泽东,陈波,廖丽..高通量微液滴技术在南大洋沉积物微生物分离培养中的应用研究[J].极地研究,2025,37(3):541-550,10.基金项目
国家重点研发计划(2022YFC2807501)资助 （2022YFC2807501）
