井冈山大学学报（自然科学版）2025，Vol.46Issue(5)：44-55,12.DOI:10.3969/j.issn.1674-8085.2025.05.006
青牛胆对阿尔茨海默病果蝇模型的抗炎与自噬保护作用研究
PROTECTIVE EFFECTS OF TINOSPORA SAGITTATA ON AUTOPHAGY AND ANTI-INFLAMMATION IN DROSOPHILA MODELS OF ALZHEIMER'S DISEASE
摘要
Abstract
The network pharmacology and molecular biology techniques were employed to investigate the therapeutic effects of Tinospora sagittata(TS)in a Drosophila model of Alzheimer's disease(AD).Ultra-performance liquid chromatography(UPLC)analysis combined with extensive literature review was used to identify the main chemical components of TS.Targets related to TS components and AD were screened using the PubChem and GeneCards databases,respectively.Protein-protein interaction(PPI)analysis was performed using the STRING database to identify key targets.The DAVID 2021 database was utilized to analyze the biological processes and pathways involved.Behavioral and molecular experiments were conducted for validation.The results showed that TS contains 54 main active components,1,078 potential targets,and 2,385 AD-related targets,with 404 overlapping genes identified.Gene Ontology(GO)functional enrichment analysis revealed 1,771 items,including 1,327 biological processes(BP),169 cellular components(CC),and 275 molecular functions(MF).Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis identified 189 pathways.Core targets of TS against AD,such as PIK3CA,PIK3CB,and EGFR,were screened.Western blot analysis confirmed that TS downregulated the expression of core targets PIK3CA and PIK3CB,promoted autophagy by inhibiting the AKT/mTOR signaling pathway,and reduced the levels of NF-κB and inflammatory factors IL-2 and IL-6,thereby exerting anti-AD effects.This study provides a theoretical foundation for the application of TS in the treatment of Alzheimer's disease and offers new insights into the rapid screening and validation of anti-AD natural drugs using the Drosophila model.关键词
青牛胆/阿尔茨海默病/果蝇/抗炎/自噬Key words
Tinospora sagittata/Alzheimer's disease/Drosophila melanogaster/anti-inflammatory/autophagy分类
生物科学引用本文复制引用
毋肇琪,曹进国..青牛胆对阿尔茨海默病果蝇模型的抗炎与自噬保护作用研究[J].井冈山大学学报（自然科学版）,2025,46(5):44-55,12.基金项目
国家自然科学基金项目(32160177) （32160177）
