井冈山大学学报（自然科学版）2025，Vol.46Issue(5)：62-68,7.DOI:10.3969/j.issn.1674-8085.2025.05.008
α-红没药醇抑制油茶炭疽病病菌的机理研究
THE MECHANISM OF α-BISABOLOL IN INHIBITING ANTHRACNOSE IN CAMELLIA OLEIFERA
摘要
Abstract
This study took α-bisabolol as the research object.Through conducting the mycelial growth inhibition test of the Colletotrichum gloeosporioides(the pathogen causing anthracnose of Camellia.oleifera),and combining with the analysis of indicators such as observing the morphological changes of mycelia by scanning electron microscopy and measuring the cell membrane permeability by the electrical conductivity method,the antibacterial mechanism was explored in depth.The results showed that the minimum inhibitory concentration(MIC)of this compound was 0.050 00 μL/mL,and it could inhibit the pathogenic bacteria through the synergistic effect of multiple pathways.Electrical conductivity increased 5.289 70-fold after 12 h,indicating the damage of cell membrane,while scanning electron microscopy revealed severe mycelial shrinkage and deformation;the oxidative stress system was disrupted as superoxide dismutase(SOD)activity peaked at 3.186 00 times the control then declined sharply,and catalase(CAT)activity increased to 1.865 30 U/mg prot;metabolic function was significantly affected with soluble protein leakage 3.584 10-fold higher than the control,reduced soluble sugar content,and a 93.4％decrease in Ca++Mg++-ATPase activity.Therefore,α-bisabolol can exert antibacterial effects through multiple-target mechanisms such as damaging cell membrane structure,disrupting oxidative balance,and inhibiting energy metabolism,it would provide a theoretical support for the development of plant-derived bactericides for the prevention and control anthracnose of C.oleifera.关键词
α-红没药醇/油茶炭疽病防治/胶孢炭疽菌/抑菌机理Key words
α-bisabolol/control of anthracnose of Camellia oleifera/Colletotrichum gloeosporioides/antifungal mechanism分类
农业科技引用本文复制引用
胡文杰,俞静怡,罗辉,卓志嘉,周兵,彭舒军,殷帅文..α-红没药醇抑制油茶炭疽病病菌的机理研究[J].井冈山大学学报（自然科学版）,2025,46(5):62-68,7.基金项目
国家自然科学基金项目(32160089) （32160089）
江西省自然科学基金资助面上项目(20232BAB205044,20181BAB204014,20252BAC240486) （20232BAB205044,20181BAB204014,20252BAC240486）
江西省教育厅科技研究重点项目(GJJ2201609) （GJJ2201609）
国家级大学生创新训练计划项目(202510419008) （202510419008）
