江苏大学学报（医学版）2025，Vol.35Issue(5)：369-375,7.DOI:10.13312/j.issn.1671-7783.y240112
紫草素脂质体的制备及其体内外评价
Preparation of shikonin-liposomes and its in vivo-in vitro evaluation of anti-aging
摘要
Abstract
Objective:To prepare shikonin liposomes(SHK-L)and preliminarily evaluate their anti-aging effects.Methods:The SHK-L prescription was optimized through single factor experiments to investigate its in vitro release and oral bioavailability in rats.The in vivo safety was evaluated through zebrafish toxicity experiments,and the anti-aging effect was examined by staining the β-galactosidase in aging zebrafish.Results:The optimal prescription was SHK 6 mg,lecithin 70 mg,cholesterol 10 mg,vitamin E polyethylene glycol succinate(TPGS)30 mg,and sodium dodecyl sulphate(SDS)1.5 mg,with a particle size of(206.96±0.75)nm,polydispersity coefficient(0.186±0.030),encapsulation rate of(96.88±2.19)%and drug loading was(5.36±0.01)%.SHK-L improved the drug release rate compared to SHK.According to in vivo pharmacokinetic data,SHK-L had a relative bioavailability of 413.37%of SHK.At 96 hours post-administration,LC50 of shikonin and SHK-L in zebrafish were(0.112±0.007)μg/mL and(0.141±0.012)μg/mL,respectively.Conclusion:SHK-L can enhance the solubility and oral bioavailability of insoluble drugs,as well as their anti-aging effect and safety.关键词
紫草素/脂质体/体外释放/口服生物利用度/抗衰老/安全性Key words
shikonin/liposomes/in vitro release/oral bioavailability/anti-aging/safety分类
医药卫生引用本文复制引用
翟颖,余江南,徐希明..紫草素脂质体的制备及其体内外评价[J].江苏大学学报（医学版）,2025,35(5):369-375,7.基金项目
国家自然科学基金资助项目(8217131836) （8217131836）
