江苏大学学报（医学版）2025，Vol.35Issue(5)：381-386,6.DOI:10.13312/j.issn.1671-7783.y240222
双联益生菌水凝胶的制备及其选择性抑菌和清除活性氧效果评价
Synthesis of a dual-probiotic hydrogel and evaluation of its selective antibacterial and reactive oxygen species scavenging effects
摘要
Abstract
Objective:To develop a wound dressing incorporating Clostridium butyricum(C.butyricum,anaerobic probiotic),which is capable of producing antibacterial substances and exerting hydrogen-producing properties under normoxic conditions,and investigate the effects of its selective bacteria inhibition and reactive oxygen species(ROS)scavenging.Methods:C.butyricum and Bacillus subtilis(B.subtilis,aerobic probiotic)were simultaneously encapsulated in hydrogel to prepare a dual-probiotic hydrogel(BC-gel).B.subtilis was used to consume oxygen within the hydrogel.This creates an anaerobic microenvironment for C.butyricum to maintain its viability.The selective bacteria inhibition of BC-gel was evaluated by plate counting method,while the selective scavenging of ROS was evaluated through flow cytometry.Results:BC-gel can effectively inhibit the proliferation of Escherichia coli and methicillin-resistant Staphylococcus aureus.While maintaining the normal growth of Bifidobacterium and Lactobacillus,BC-gel has the ability to scavenge·OH and ONOO-free radicals,but has no significant decreasing effect on O2-·and NO·.Conclusion:BC-gel exhibits favorable selective bacteria inhibition and ROS scavenging properties.关键词
糖尿病溃疡/创面修复/丁酸梭菌/枯草芽孢杆菌/抗菌/活性氧/双联益生菌水凝胶Key words
diabetic ulcer/wound repair/Clostridium butyricum/Bacillus subtilis/antibacterial/reactive oxygen species/dual-probiotic hydrogel分类
医药卫生引用本文复制引用
陈嘉玲,黄钰文,陆科承,何雪婷,章马旭阳,沈海俊..双联益生菌水凝胶的制备及其选择性抑菌和清除活性氧效果评价[J].江苏大学学报（医学版）,2025,35(5):381-386,6.基金项目
江苏高校"青蓝工程"资助(2022年) （2022年）
